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Position: Home > Articles > Effect of Dandelion Extract on Inflammatory Cytokine Secretions in LPS-stimulated Mouse Macrophages Progress in Veterinary Medicine 2011,32 (2) 49-51

蒲公英提取物对LPS激活小鼠腹腔巨噬细胞炎症因子分泌的影响

作  者:
刘利本;平家奇;刘婧陶;张雪梅
单  位:
延边大学农学院动物医学系
关键词:
蒲公英提取物;炎症因子;脂多糖
摘  要:
采用MTT法检测蒲公英提取物对LPS激活小鼠腹腔巨噬细胞的毒性作用,并应用ELISA法测定蒲公英提取物对脂多糖LPS激活小鼠腹腔巨噬细胞TNF-α、IL-6和IL-1β的含量。结果表明,蒲公英提取物在≤1 mg/mL剂量范围内对巨噬细胞无细胞毒作用,对LPS刺激的小鼠腹腔巨噬细胞TNF-α,IL-6和IL-1β分泌具有明显的抑制作用,且呈一定的剂量依赖关系。
译  名:
Effect of Dandelion Extract on Inflammatory Cytokine Secretions in LPS-stimulated Mouse Macrophages
作  者:
LIU Li-ben,PING Jia-qi,LIU Jing-tao,ZHANG Xue-mei(Department of Veterinary Medicine,Agricultural College of Yanbian University,Longjing,Jilin,133400,China)
关键词:
dandelion extract;inflammatory factor;LPS
摘  要:
The cytotoxic activity of dandelion extract on lipopolysaccharide(LPS)-stimulated mouse macrophages was examined by using MTT methods,the infammatory cytokine secretions in LPS-stimulated macrophages were determined by ELSA method.The results showed that dandelion extract was not cytotoxic on LPS-stimulated macrophages in less than or equal to 1 mg/mL.The dandelion extract has obvious inhibitory effect on infammatory cytokine secretions in a dose-dependent manner in LPS-stimulated mouse macrophages.

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