中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 高效液相色谱-蒸发光散射检测法测定茶叶中单糖和双糖 茶叶科学 2010,30 (6) 435-439
Position: Home > Articles > Determination of Monosaccharides and Disaccharides in Tea by High Performance Liquid Chromatography-Evaporative Light Scattering Detector Journal of Tea Science 2010,30 (6) 435-439

高效液相色谱-蒸发光散射检测法测定茶叶中单糖和双糖

作  者：
袁勇;黄建安;李银花;陈金华;袁玲;刘仲华
单  位：
湖南农业大学茶学教育部重点实验室国家植物功能成分利用工程技术研究中心
关键词：
高效液相色谱;蒸发光散射检测器;茶叶;单糖;双糖
摘  要：
建立了高效液相色谱-蒸发光散射检测器(HPLC-ELSD)测定茶叶中单糖和双糖的检测方法。采用ShodexNH2P-50-4E(4.6mm×250mm,5μm)色谱柱,以水和乙腈为流动相进行梯度洗脱,梯度洗脱程序为:0～14min,85%乙腈;14～16min,85%～70%乙腈;16～28min,70%乙腈;28～32min,70%～85%乙腈,流速:1.0mL/min,柱温:20℃,雾化管温度为30℃,漂移管温度为70℃,氮气压力为310.275Pa。鼠李糖、阿拉伯糖、木糖、果糖、甘露糖、葡萄糖、蔗糖、麦芽糖在3.202～184.828μg范围内程良好的线性关系,8种糖的加标回收率在90.97%～105.07%之间,最低检测限(S/N=3)范围在6.976～1376.297ng。该方法具有操作简便,分离效果好等特点。
译  名：
Determination of Monosaccharides and Disaccharides in Tea by High Performance Liquid Chromatography-Evaporative Light Scattering Detector
作  者：
YUAN Yong,HUANG Jian-an,LI Yin-hua,CHEN Jin-hua,YUAN Ling,LIU Zhong-hua (Key Lab of Tea Science of Ministry of Education,Hunan Agricultural University,National Research Center of Engineering & Technology for Utilization of Functional Ingredients from Botanical,Changsha 410128,China)
关键词：
HPLC,ELSD,tea,monosaccharides,disaccharides
摘  要：
A method for determination of monosaccharide and disaccharide in tea by HPLC-ELSD was developed. Shodex NH2P-50-4E(4.6 mm×250 mm,5μm) column was used at 20℃,acetonitrile and water used as mobile phase,for gradient elusion with the flow rate of 1.0 mL/min.The ratio of organic solvent was controlled by a gradient elution procedure.The procedure was as follows:85%acetonitrile in 0～14 min,70%～80%acetonitrile 14～16 min,70%acetonitrile 16～28 min,then 70%～80%acetonitrile 28～32 min.At last,the ratio of acetonitrile increased to 85%. With the use of evaporative light-scattering detector,the nebulization chamber temperature was 30℃,the drift tube temperature was 70℃and nitrogen pressure was 310.275 Pa.The calibration curves of rhamnose,arabinose,xylose,fructose,mannose,glucose,sucrose and maltose showed good linearity in the range of 3.202～184.828μg.The recoveries of fortified 8 kinds of sugar were 90.97%～105.07%,limit of detention was 6.976～1 376.297 ng.Results indicated that the method was simple,rapid and could effectively separate the target component.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46