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Position: Home > Articles > Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography-Evaporative Light-Scattering Detector Journal of Tea Science 2005,25 (3) 225-228

高效液相色谱-蒸发光散射检测器测定茶叶中茶氨酸

作  者:
李银花;刘仲华;黄建安;龚雨顺
单  位:
湖南农业大学天然产物研究中心
关键词:
高效液相色谱;蒸发光散射检测器;茶氨酸;茶叶
摘  要:
建立未衍生化高效液相色谱-蒸发光散射检测器测定茶叶中茶氨酸。采用Polaris-C18(250mm×4.6mm)色谱柱,以含0.1%(体积分数)三氟乙酸的水和0.1%(体积分数)三氟乙酸的乙腈作梯度洗脱,流速0.5ml/min,蒸发光散射检测器为ELSD-2000。茶氨酸浓度在0.062~0.450mg/ml内,其峰面积的常用对数与进样量的常用对数呈良好的线性关系,平均回收率为97.5%。本方法具有精确、灵敏等特点。
译  名:
Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography-Evaporative Light-Scattering Detector
作  者:
LI Yin-hua,LIU Zhong-hua*,HUANG Jian-an,GONG Yu-shun(Institute of Natural Products,Hunan Agricultural University,Changsha 410128,China)
关键词:
HPLC,Evaporative light-scattering detector,Theanine,Tea
摘  要:
High performance liquid chromatography (HPLC) coupled with evaporative light scattering detector (ELSD-2000)was used for the determination of theanine in tea without derivatization.A Polaris-C18 column (250 mm×4.6 mm) was used with the mobile phase of 0.1%trifluoroacetic acid water solution and 0.1% trifluoroacetic acid acetonitrile solution.The flow rate was at 0.5 ml/min.The linear response ranged from 0.062~0.450 mg/mL for theanine.The recovery was 97.5%.The method was evaluated as accurate and simple.

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