中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 转基因水稻深加工产品两种荧光定量方法的比较研究 东北农业大学学报 2011,42 (1) 40-45
Position: Home > Articles > Comparison of two real-time PCR techniques for quantification of GMO contents in highly processed products of rice Journal of Northeast Agricultural University 2011,42 (1) 40-45

转基因水稻深加工产品两种荧光定量方法的比较研究

作  者：
敖金霞;高学军;权东升;徐迪;于艳波;李璐;袁肖寒
单  位：
东北农业大学生命科学与生物技术研究中心;黑龙江省家畜繁育指导站
关键词：
转基因水稻;深加工制品;实时荧光定量PCR;TaqMan;SYBR GreenⅠ
摘  要：
根据转基因水稻中外源基因Cry1A(B)和内源基因SPS设计SYBR GreenⅠ引物、TaqMan引物和荧光探针,以内源基因SPS作为内参照,使用两种FQ-PCR方法对水稻深加工产品中转基因水稻的含量进行定量检测。建立了转基因水稻标准品Cry1A(B)和SPS之间的△Ct与样品中转基因水稻含量百分比之间的校正曲线和线性回归方程,并对4种未知水稻深加工样品进行检测,同时比较两者的检测结果。结果表明,TaqMan和SYBR GreenⅠ两种荧光定量PCR检测技术的检测结果可靠,且均可用于转基因深加工产品的检测。
译  名：
Comparison of two real-time PCR techniques for quantification of GMO contents in highly processed products of rice
作  者：
AO Jinxia1,GAO Xuejun1,QUAN Dongsheng2,XU Di2,YU Yanbo1,LI Lu1,YUAN Xiaohan1(1.Life Science and Biotechnique Research Center,Northeast Agricultural University,Harbin 150030,China;2.Livestock Breeding Station Guide of Heilongjiang Province,Harbin 150060,China)
关键词：
transgenic rice;highly processed products;real-time PCR;TaqMan;SYBR Green Ⅰ
摘  要：
Transgenic rice was quantitatively detected by ABI Prism 7300 sequence detector with the PCR primers and fluorescence probes designed according to the sequences of endogenous SPS gene and exogenous Cry1A(B) gene,and the PCR systems were based on SYBR Green Ⅰ and TaqMan.The standard curve of △Ct between Cry1A(B) gene and SPS gene of transgenic rice in standard materials were generated and a linear regression equation was obtained.Quantification methods were optimized through two different real-time PCR chemistries,i.e.SYBR Green Ⅰ and TaqMan,and the transgenic rice contents were quantified in five standard samples and four highly processed products by two assays.Both methods are proved to be specific,highly sensitive and reliable for both identification and quantification of rice DNA.The results showed that the two optimized PCR systems could be used for practical quantitative detection of transgenic rice in highly processed products.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46