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Position: Home > Articles > Determination of ATP-Related Compounds in Fish by RP-HPLC MEAT RESEARCH 2012 (4) 24-27

反相高效液相色谱法检测鱼肉中的ATP关联物

作  者：
贾鹏禹;孙蕊;俞龙浩;何淑清;张盟;张宇宁
单  位：
黑龙江八一农垦大学动物科技学院;黑龙江八一农垦大学食品学院;黑龙江八一农垦大学
关键词：
高效液相色谱;ATP关联物;鱼肉
摘  要：
建立同时测定鱼肉组织中6种ATP关联物的高效液相色谱方法。采用反相高效液相色谱分离模式,使用Sepax Bio-C18(250mm×4.6mm,5μm,200A。)色谱柱,以60mmol/L磷酸二氢钾、60mmol/L磷酸氢二钾组成的缓冲液(pH6.68)为流动相等比洗脱,流速0.6mL/min;紫外检测波长为254nm,带宽为16nm。6种组分在20min内基线分离,平均加标回收率在98.3%～104.1%之间,线性范围为1～400mg/L,检出限在15～40μg/L之间。该方法简便、准确,易于移植。
译  名：
Determination of ATP-Related Compounds in Fish by RP-HPLC
作  者：
JIA Peng-yu1,SUN Rui2,YU Long-hao3,,HE Shu-qing3,ZHANG Meng3,ZHANG Yu-ning3(1.Agri-Food Processing and Engineering Technology Research Center of Heilongjiang Province,Heilongjiang Bayi Agricultural University,Daqing 163319,China;2.College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319,China;3.College of Food Science,Heilongjiang Bayi Agricultural University,Daqing 163319,China)
关键词：
high performance liquid chromatography(HPLC);ATP-related compounds;fish
摘  要：
The method for determination of ATP-related compounds in fish was established.The content of ATP-related compounds in fish were determined by RP-HPLC with a Sepax Bio-C18(250mm×4.6mm ID,5μm,200 A.)column,and UV detector at a wave length of 254 nm(BW 16 nm).Mobile phase was 60 mmol/L KH2PO4 and 60 mmol/L K2HPO4(pH=6.68) and flow rate was 0.6mL/min.The separation was achieved in 20 min.All the calibration curves were linear in the mass concentration range of 1-400 mg/L.The average recoveries of the standard additions were between 98.3%-104.1%,and the LOD were between 15-40μg /L for ATP-related compounds.This method proved to be reliable and reprodcible.

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