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当前位置: 首页 > 文章 > 无血清条件下UC-MSCs和BMECs共培养对IGF-Ⅰ表达的影响 中国畜牧兽医 2017,44 (2) 411-416
Position: Home > Articles > Effect of Umbilical Cord Mesenchymal Stem Cells and Bovine Mammary Gland Epithelial Cells Co-culture under the Serum-free Condition on Expression of IGF-Ⅰ China Animal Husbandry & Veterinary Medicine 2017,44 (2) 411-416

无血清条件下UC-MSCs和BMECs共培养对IGF-Ⅰ表达的影响

作  者：
赵艳坤;邵伟;雒诚龙;余雄
单  位：
新疆农业大学动物科学学院新疆肉乳用草食动物营养实验室
关键词：
脐带间充质干细胞;奶牛乳腺上皮细胞;无血清;共培养;类胰岛素样生长因子-Ⅰ;实时荧光定量PCR
摘  要：
试验旨在探讨无血清条件下脐带间充质干细胞(UC-MSCs)和奶牛乳腺上皮细胞(BMECs)共培养对类胰岛素样生长因子-Ⅰ(IGF-Ⅰ)表达的影响,将UC-MSCs和BMECs按1∶2直接共培养,对比单纯培养的两种细胞,48h时利用ELISA法检测各组上清IGF-Ⅰ浓度水平,再利用Transwell小室将两种细胞分离培养,实时荧光定量PCR检测各组细胞IGF-ⅠR、IGF-ⅠmRNA的表达。结果显示,共培养中IGF-Ⅰ浓度显著高于UC-MSCs组(P<0.05),极显著高于BMECs组(P<0.01);UC-MSCs/BMECs、BMECs/UC-MSCs组IGF-ⅠmRNA表达值均极显著高于单纯培养组(P<0.01),BMECs/UC-MSCs组显著高于UC-MSCs/BMECs组(P<0.05);UC-MSCs/BMECs、BMECs/UC-MSCs组IGF-ⅠR mRNA表达值显著或极显著高于单纯培养组(P<0.05;P<0.01),BMECs/UC-MSCs组较UCMSCs/BMECs组差异显著(P<0.05)。综上,体外无血清培养条件下,UC-MSCs和BMECs共培养可显著提高IGF-ⅠR及IGF-ⅠmRNA表达,IGF-Ⅰ浓度水平和IGF-ⅠR mRNA的表达具有一致性,IGF-Ⅰ主要存在于UCMSCs中。
译  名：
Effect of Umbilical Cord Mesenchymal Stem Cells and Bovine Mammary Gland Epithelial Cells Co-culture under the Serum-free Condition on Expression of IGF-Ⅰ
作  者：
ZHAO Yan-kun;SHAO Wei;LUO Cheng-long;YU Xiong;Xinjiang Meat Emulsions with Plant-eating Animal Nutrition Laboratory,College of Animal Science,Xinjiang Agricultural University;
关键词：
umbilical cord mesenchymal stem cells(UC-MSCs);;bovine mammary epithelial cells(BMECs);;serum free;;co-culture;;IGF-Ⅰ;;Real-time PCR
摘  要：
The study was aimed to explore the effect of umbilical cord mesenchymal stem cells(UC-MSCs)and bovine mammary gland epithelial cells(BMECs)under the serum-free co-culturing condition on expression of IGF-Ⅰ.UC-MSCs and BMECs were co-cultured directly at the concentration ratios of 1∶2,in control groups,UC-MSCs and BMECs were cultured alone.Using ELISA method to detect the IGF-Ⅰlevels in each group supernatant at 48 h,and two kinds of cells were separated by Transwell Chambers,the IGF-Ⅰ and IGF-ⅠR mRNA expression values of each group were estimated with Real-time PCR.The results showed that the IGF-Ⅰ concentration of UC-MSCs and BMECs mixed co-culture was significantly higher than the UC-MSCs group(P<0.05),and extremely significantly higher than the BMECs group(P<0.01);The IGF-Ⅰ mRNA expression of UC-MSCs/BMECs and BMECs/UC-MSCs groups were extremely significantly higher than the control group(P<0.01),and the IGF-Ⅰ mRNA expression ofBMECs/UC-MSCs group was significantly higher than the UC-MSCs/BMECs group(P<0.05);The IGF-ⅠR mRNA expression of UC-MSCs/BMECs and BMECs/UC-MSCs groups were higher than the control group(P<0.05;P<0.01),BMECs/UC-MSCs group had significant difference compared with UC-MSCs/BMECs group(P<0.05).Conclusively,the co-culture of UCMSCs and BMECs was able to improve the IGF-ⅠR and IGF-Ⅰ mRNA expression under the serum-free condition in vitro,and the IGF-Ⅰ concentration level was correspondence with the IGF-ⅠR mRNA expression,IGF-Ⅰmainly existed in UC-MSCs.

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