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当前位置: 首页 > 文章 > 移植脐带间充质干细胞对荷斯坦公犊体重及血清免疫和抗氧化水平的影响 动物营养学报 2015,27 (2) 616-622
Position: Home > Articles > Effects of Umbilical Cord Mesenchymal Stem Cells Transplantation on Body Weight,Serum Immune and Antioxidant Levels of Holstein Bull Calves Chinese Journal of Animal Nutrition 2015,27 (2) 616-622

移植脐带间充质干细胞对荷斯坦公犊体重及血清免疫和抗氧化水平的影响

作  者：
赵艳坤;王立文;彭国亮;邵伟;余雄
单  位：
新疆肉乳用草食动物营养实验室;新疆农业大学动物科学学院
关键词：
脐带间充质干细胞;荷斯坦公犊;免疫;抗氧化;平均日增重
摘  要：
本文旨在探究移植脐带间充质干细胞(UC-MSCs)对荷斯坦公犊体重及血清免疫和抗氧化水平的影响。选取1月龄体重相近的荷斯坦公犊28头,随机分为对照组和试验组,每组14头。采集健康的荷斯坦新生胎牛的脐带组织,通过胰酶消化筛选法体外分离、纯化培养UC-MSCs,试验组公犊颈静脉注射UC-MSCs 3×105个/kg BW(稀释至8 m L),对照组注射等量生理盐水,分别与1、2、3、4、5月龄各注射1次。试验期150 d。结果表明:与对照组相比,公犊移植UC-MSCs,1)显著提高4、5、6月龄体重(P<0.05);显著提高3、4月龄(P<0.05),极显著提高6月龄(P<0.01)的平均日增重;2)极显著提高4月龄(P<0.01),显著提高6月龄(P<0.05)血清免疫球蛋白A(Ig A)含量;极显著提高2、5月龄(P<0.01),显著提高6月龄(P<0.05)血清免疫球蛋白G(Ig G)含量;极显著降低3月龄(P<0.01),显著降低4月龄(P<0.05)血清白细胞介素2(IL-2)含量;3)极显著降低2、3、4、5、6月龄血清丙二醛(MDA)含量(P<0.01);显著提高5月龄(P<0.05),极显著提高6月龄(P<0.01)血清超氧化物歧化酶(SOD)活性。由此可见,移植UC-MSCs能够提高公犊血清Ig A、Ig G含量;降低血清MDA含量,提高SOD活性,增强机体清除自由基的能力;降低血清IL-2含量,减少内部炎症发生;从而提高公犊机体免疫力,进而促进公犊的生长发育。
译  名：
Effects of Umbilical Cord Mesenchymal Stem Cells Transplantation on Body Weight,Serum Immune and Antioxidant Levels of Holstein Bull Calves
作  者：
ZHAO Yankun;WANG Liwen;PENG Guoliang;SHAO Wei;YU Xiong;Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition,College of Animal Science,Xinjiang Agricultural University;
关键词：
umbilical cord mesenchymal stem cells;;Holstein bull calves;;immunity;;antioxidant;;average daily gain
摘  要：
This article aimed to explore the effects of umbilical cord mesenchymal stem cells(UC-MSCs)transplantation on body weight(BW),serum immune and antioxidant levels of Holstein bull calves.Twenty eight 1-month-old Holstein bull calves with similar body weight were selected and randomly divided into control and experimental groups with 14 calves per group.Fresh fetal umbilical cord tissue was collected from healthy newborn Holstein calves.UC-MSCs were purified and cultivated in vitro by the method of pancreatic enzyme digestion.Calves of experimental group were injected in jugular vein with 3 ×105cells / kg BW UC-MSCs(diluted to 8 m L),while those of control group were injected with normal saline at equal dose.The injection was carried out at 1,2,3,4 and 5 months of age,respectively.The test lasted for 150 d.The results showed as follows: compared with control group,the transplantation of UC-MSCs in bull calves,1) significantly increased BW at 4,5 and 6 months of age(P < 0.05); significantly increased average daily gain at 3(P<0.05),4(P<0.05) and 6 months of age(P < 0.01); 2) significantly increased serum immunoglobulin A(Ig A) content at 4(P<0.01) and 6 months of age(P<0.05); significantly increased serum immunoglobulin G(Ig G) at 2(P<0.01),5(P<0.01) and 6 months of age(P<0.05); significantly decreased serum interleukin-2(IL-2) content at 3(P<0.01) and 4 months of age(P < 0.05); 3) significantly decreased serum malondialdehyde(MDA) content at 2,3,4,5 and 6 months of age(P<0.01); significantly increased serum superoxide dismutase(SOD) activity at 5(P<0.05) and 6 months of age(P<0.01).Thus,the transplantation of UC-MSCs can increase serum Ig A and Ig G contents,decrease serum MDA content,increase serum SOD activity,enhance the body 's ability to clear free radicals,reduced serum IL-2 content,and reduced internal inflammation,thereby enhance immunity,and stimulate the growth and development of bull calves.

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