当前位置: 首页 > 文章 > 奶牛瑟氏泰勒虫P33表面蛋白基因的克隆及序列分析 黑龙江畜牧兽医 2008 (3) 15-17
Position: Home > Articles > Cloning and sequence analysis of the dairy cow Theileria sergenti P33 surface protein gene Heilongjiang Animal Science and Veterinary Medicine 2008 (3) 15-17

奶牛瑟氏泰勒虫P33表面蛋白基因的克隆及序列分析

作  者:
曹世诺;于龙政;薛书江;贾立军;柴方红;张守发
单  位:
延边大学农学院动物医学系
关键词:
牛瑟氏泰勒虫;P33表面蛋白基因序列;克隆;序列分析
摘  要:
研究根据GenBank上发表的牛瑟氏泰勒虫P33表面蛋白的基因序列设计合成1对引物,用PCR方法扩增出了牛瑟氏泰勒虫的基因片段,将该基因纯化后连接到pMD18-T载体上,进行序列测定和分析,并用此方法对延边某奶牛场10头奶牛进行了检测。结果表明:10份样品中9份为阳性,所扩增的目的基因核苷酸长为868bp,共编码283个氨基酸;该段基因片段与牛泰勒虫韩国株核苷酸序列同源性为99·4%,氨基酸同源性为99·3%。
译  名:
Cloning and sequence analysis of the dairy cow Theileria sergenti P33 surface protein gene
作  者:
CAO Shi-nuo,YU Long-zheng,ZHANG Shou-fa,et al(Department of Veterinary Medicine,Yanbian University,Longjing 133400,China)
关键词:
Theileria sergenti ;P33 surface protein;cloning;sequencing analysis
摘  要:
A pair of primers was designed according to the T.S.gene sequences published in the GenBank.The T.S.gene strain was amplified by PCR.The purified templates DNA were cloned into pMD18-T vector.The method was used to detect ten cows in the dairy farm.Nine in ten were positive by sequencing aralysis of the T.S.gene.The fragment size was 868 bp,which encodes a protein of 283 amino acids,the homology of nucleotide sequence with Korean strain was 99.4% and 99.3% at amino acid level.This results may be used for expression and studies on vaccine.

相似文章

计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊