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当前位置: 首页 > 文章 > 同位素稀释液质联用法测定鱼体中氯丙嗪的残留 海洋渔业 2011,33 (1) 85-92
Position: Home > Articles > Determination of Chlorpromazine residue in fishies by isotope dilution liquid chromatography-tandem mass spectrometry Marine Fisheries 2011,33 (1) 85-92

同位素稀释液质联用法测定鱼体中氯丙嗪的残留

作  者：
于慧娟;王庆贺;戚隽渊;惠芸华;黄冬梅;金高娃;冯兵
单  位：
中国水产科学研究院东海水产研究所;上海水产研究所
关键词：
氯丙嗪;同位素稀释;高效液相色谱-串联质谱;欧洲鳗鲡;鳜鱼;大口黑鲈
摘  要：
建立了高效液相色谱-串联质谱(HPLC/MS/MS)分析鱼体中氯丙嗪残留的方法。样品用乙腈提取,MCX固相萃取柱净化,氨化甲醇洗脱。在正离子模式下以电喷雾串联质谱仪进行测定,采用内标法定量,氘代氯丙嗪为内标物。以欧洲鳗鲡、鳜鱼和大口黑鲈为研究对象,在1.0、5.0、25.0μg/kg添加水平下,方法平均回收率为90.1%~95.1%(批内),相对标准偏差为2.5%~5.7%(批内)。方法定量限(S/N>10)为1.0μg/kg。方法检出限(S/N>3)为0.5μg/kg。实验结果表明:该方法简单、快速、准确度高、重复性好,适合于鱼类样品中氯丙嗪的残留检测。
译  名：
Determination of Chlorpromazine residue in fishies by isotope dilution liquid chromatography-tandem mass spectrometry
作  者：
YU Hui-juan1,WANG Qin-he2,QI Jun-yuan2,HUI Yun-hua1,HUANG Dong-mei1,JIN Gao-wa1,FENG Bing1(1.East China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences,Shanghai 200090,China;2.Shanghai Fisheries Research Institute,Shanghai 200433,China).
关键词：
Chlorpromazine;isotope dilution;HPLC/MS/MS;Anguilla Anguilla;Siniperca chuatsi;Micropterus salmoides
摘  要：
A method to detect chlorpromazine residues in fish products was developed by using high performance liquid chromatography-tandem mass spectrometry(HPLC/ MS/MS).Chlorpromazine was extracted with acetonitrile from the test tissue.The extract was cleaned up and concentrated on an Oasis MCX cartridge,eluted by basic methanol and then analyzed by HPLC-MS/MS.The quantification was performed with internal standard method,with D6-Chlorpromazine as internal standard.The average recoveries were 90.1%-95.1%(Intraday) in the spiked range of 1-25 μg/kg in fish.The relative standard deviations were 2.5%-5.7%(Intraday).The limits of quantification(S /N >10) were 1.0 μg/kg and the limits of detection(S /N >3) were 0.5 μg/kg.Results indicated that the method used was easier,faster and more accurate.

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