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当前位置: 首页 > 文章 > 同位素内标液质联用法测定动物源食品中的氯丙嗪残留量 热带农业科学 2018,38 (3) 104-108
Position: Home > Articles > Determination of Chlorpromazine Residues in Animal-based Food by an Isotope-labeled Internal Standard Liquid Chromatography-Tandem Mass Spectrometry Chinese Journal of Tropical Agriculture 2018,38 (3) 104-108

同位素内标液质联用法测定动物源食品中的氯丙嗪残留量

作  者：
吴基任;李成;谭高好;莫秋岸;徐彬
关键词：
超高效液相-串联质谱;同位素内标;氯丙嗪;动物源食品
摘  要：
建立测定动物源食品中氯丙嗪(Chlorpromazine)残留量的液相色谱串联质谱同位素内标方法。样品用乙腈和水(80/20)超声波提取后,用Waters Oasis PRi ME HLB小柱净化,Waters ACQUITY UPLC BEH C18色谱柱分离。以乙腈和0.1%甲酸水溶液为流动相进行梯度洗脱,用电喷雾正离子(ESI+)多反应监测、基质校准同位素内标法进行定量分析。方法在0.1~20μg/L范围内具有良好线性,相关系数r=0.998。样品在低、中、高3个浓度水平下的平均回收率为89.1%~93.5%,相对标准偏差(RSD)为2.5%~4.8%。检出限(S/N=3)为0.1μg/kg,定量下限(S/N=10)为0.3μg/kg。
译  名：
Determination of Chlorpromazine Residues in Animal-based Food by an Isotope-labeled Internal Standard Liquid Chromatography-Tandem Mass Spectrometry
作  者：
WU Jiren;LI Cheng;TAN Gaohao;MO Qiuan;XU Bin;Hainan Institute for Food Control;Sanya Division of Hainan Provincial Institute for Drug and Food Control;Danzhou Division of Hainan Provincial Institute for Drug and Food Control;
关键词：
supper-high performance liquid chromatography-tandem mass spectrometry;;isotope-labeled internal standard;;chlorpromazine;;animal-based food
摘  要：
Chlorpromazine residues in animal-based food were detected by liquid chromatography-tandem mass spectrometry and quantified by using an isotope-labeled internal standard. The samples were extracted by acetonitrile and water(80/20), purified by Waters Oasis PRi ME HLB column, separated by Waters ACQUITY UPLC BEH C18 column. The acetonitrile and 0.1% formic acid aqueous solution were used as the mobile phase for gradient elution. The chlorpromazine was detected by using electrospray ionization(ESI+) in positive ion mode with multiple reaction monitoring(MRM), and its quantity was analyzed by using the matrix matched calibration method combined with the stable isotope-labeled internal standard. This combined method had a good linearity in the range of 0.1~20 g/L, and its correlation coefficient was r=0.998. The mean recoveries of spiked samples at three levels(low, moderate and high)ranged from 89.1% to 93.5% with relative standard deviations(RSD) of 2.5%~4.8%. The detection limit(S/N=3) was 0.1 g/kg, and the lower limit of quantitation(S/N=10) was 0.3 g/kg.

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