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Position: Home > Articles > Studies on the Determination of Total Saponin of the Momordica. charantia Var. abbreviata Ser FOOD SCIENCE 2005,26 (8) 313-317

癞葡萄总皂甙测定方法的研究

作  者:
顾小红;赵海雯;汤坚;王利平
单  位:
江南大学分析测试中心;江南大学食品学院
关键词:
癞葡萄;皂甙;测定
摘  要:
研究了以花旗参皂甙为标准品,采用比色法对癞葡萄皂甙进行定量测定的方法。通过比较,确定在546nm下,以5%香草醛-冰乙酸溶液和0.8ml高氯酸溶液为显色剂、显色时间为15min、显色温度为60℃时比色体系最稳定。所得回归方程为Y=0.0039X-0.0015,线性可靠,曲线高度显著,失拟性小。方法的精密度好,相对标准差RSD=0.39%,平均回收率102.56%,标准差S=6.12,变异系数小,CV=5.97%。
译  名:
Studies on the Determination of Total Saponin of the Momordica. charantia Var. abbreviata Ser
作  者:
GU Xiao-hong1,ZHAO Hai-wen2,TANG Jian1,WANG Li-ping1(1.Analysis Center Southern Yangtze University, Wuxi 214036, China;2.School of Food Science andTechnology, Southern Yangtze University, Wuxi 214036, China)
关键词:
Momordica. charantia Var. abbreviata Ser;saponin;determination
摘  要:
A spectrophotometric method was established by using ginseng saponin as standard in 546nm for the quantitativeanalysis of the total saponins in the Momordica. charantiaV ar.a bbreviata Ser. Colorimetric system was established that the samplereacted with 5% vanillin-acetic acid glacial solution and 0.8ml the perchloric acid at 60℃ for 15min, then it turned to amaranth.The analytical precision was good, RSD was 0.39%, the average recovery was 102.56%, the standard error was 6.12, CV was 5.97%.

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