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当前位置: 首页 > 文章 > 一株同时降解玉米赤霉烯酮和黄曲霉毒素B1的谷氨酸棒状杆菌及其降解特性研究 饲料工业 2019,40 (2) 34-39
Position: Home > Articles > Isolation of a corynebacterium glutamate capable of degrading of ZEN and AFB1, and investigation of its degrading characteristics Feed Industry 2019,40 (2) 34-39

一株同时降解玉米赤霉烯酮和黄曲霉毒素B1的谷氨酸棒状杆菌及其降解特性研究

作  者：
唐彧;张琼琼;郭永鹏;郑雅文;赵丽红
单  位：
动物营养学国家重点实验室中国农业大学动物科技学院
关键词：
真菌毒素;玉米赤霉烯酮;黄曲霉毒素B1;谷氨酸棒状杆菌;生物降解
摘  要：
试验旨在筛选同时降解玉米赤霉烯酮(ZEN)和黄曲霉毒素B(1AFB1)的菌株,并研究其对两种毒素的降解特征.从土壤及鸡、驴的食糜中筛选出9株可同时降解ZEN和AFB1的菌株;编号为406的菌株经形态学观察和16S rDNA鉴定为谷氨酸棒状杆菌(Corynebacterium glutamate).谷氨酸棒状杆菌406降解ZEN和AFB1活性成分存在于发酵上清液中,其对ZEN和AFB1的降解率分别为50%和58%.通过热、胰蛋白酶、十二烷基硫酸钠(SDS)处理,初步确定降解活性物质为一种胞外酶.上清液对ZEN和AFB1的最适降解温度为67 ℃,最适pH值为8.本研究筛选到的同时降解ZEN和AFB1的谷氨酸棒状杆菌菌株406为食品和饲料中ZEN和AFB1的生物降解提供菌种资源.
译  名：
Isolation of a corynebacterium glutamate capable of degrading of ZEN and AFB1, and investigation of its degrading characteristics
作  者：
Tang Yu;Zhang Qiongqiong;Guo Yongpeng;Zheng Yawen;Zhao Lihong;
关键词：
mycotoxins;;zearalenone;;aflatoxin B_1;;corynebacterium glutamate;;biodegradation
摘  要：
The aim of this study was to screen bacterial isolates capable of simultaneously degrading zearalenone(ZEN) and aflatoxin B_1(AFB_1) and to preliminarily study the degradation characteristics.Bacterial strains were isolated from soil and chyme of chicken and donkey, and nine strains were found to be able to effectively degrade both ZEN and AFB_1. The strain named 406 was identified as a Corynebacterium glutamate by morphological observation and 16 SrDNA sequence analysis. The ZEN and AFB_1 degrading activity was predominantly attributed to fermentation supernatant of Corynebacterium glutamate 406 and the degradation ratio of ZEN and AFB_1 was 50% and 58% respectively. The fermentation supernatant of 406 was treated with heating, trypsin and sodium dodecyl sulfate(SDS) to determine active components, indicating that extracellular enzyme(s) were responsible for the degradation of ZEN and AFB_1. The optimal temperature for the maximal degradation of ZEN and AFB_1 was 67 ℃and the optimal initial pH was 8. Corynebacterium glutamate 406 capable of simultaneously degrading ZEN and AFB_1 might be a promising candidate for ZEN and AFB_1 biodegradation in food and feed.

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