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当前位置: 首页 > 文章 > 广西巴马小型猪PAQR3基因克隆及表达谱分析 南方农业学报 2016,47 (8) 1390-1395
Position: Home > Articles > Cloning and expression patterns analysis of Guangxi Bama mini-pig PAQR3 gene Journal of Southern Agriculture 2016,47 (8) 1390-1395

广西巴马小型猪PAQR3基因克隆及表达谱分析

作  者：
司景磊;黄玥萌;李龙;陈秋明;严雪瑜;吴延军;张笠;蒋钦杨;兰干球;郭亚芬
单  位：
广西大学动物科学技术学院
关键词：
广西巴马小型猪;PAQR3基因;克隆;表达谱分析
摘  要：
【目的】克隆广西巴马小型猪孕激素和脂联素分子受体3(PAQR3)基因,并研究该基因在广西巴马小型猪不同组织中的表达特性,为揭示PAQR3的功能及其在疾病模型中的作用打下基础。【方法】采用RT-PCR扩增广西巴马小型猪PAQR3基因,应用在线预测软件对其功能和结构进行生物信息学分析,并以q RT-PCR分析PAQR3基因在不同组织中的表达特性及高脂高糖诱导后的表达水平。【结果】广西巴马小型猪PAQR3基因编码区(CDS)序列全长936 bp,编码312个氨基酸,与人类(XM_006714106.2)、猕猴(NM_001257693.1)、牛(XM_010806259.1)、家犬(XM_014109889.1)、家鼠(XM_006534874.2)、羊(XM_012180242.1)、鸡(XM_001233720.3)PAQR3氨基酸序列的一致性在81%以上,其中与猕猴的亲缘关系最近,与牛的亲缘关系相对较远。PAQR3蛋白氨基酸序列包含保守的Hly IⅡ结构域,为亲水性蛋白,有7个跨膜螺旋结构;其二级结构主要是延伸链,占31.83%;该蛋白不存在信号肽,不属于分泌蛋白。q RT-PCR分析结果表明,PAQR3基因在广西巴马小型猪的不同组织中均有表达,以在肺脏中的表达量最高、脂肪中的表达量最低;经高脂高糖诱导后,PAQR3基因在广西巴马小型猪肝脏组织中的表达水平显著降低(P<0.05)。【结论】PAQR3基因在广西巴马小型猪不同组织中广泛表达,在胆固醇和甘油三脂充裕的条件下,机体可通过下调PAQR3基因的表达量来平衡胆固醇含量。
译  名：
Cloning and expression patterns analysis of Guangxi Bama mini-pig PAQR3 gene
作  者：
SI Jing-lei;HUANG Yue-meng;LI Long;CHEN Qiu-ming;YAN Xue-yu;WU Yan-jun;ZHANG Li;JIANG Qin-yang;LAN Gan-qiu;GUO Ya-fen;College of Animal Science and Technology,Guangxi University;
关键词：
Guangxi Bama mini-pig;;PAQR3 gene;;cloning;;expression patterns analysis
摘  要：
【Objective】The present experime nt was conducted to clone progesterone and adipo Q receptor 3(PAQR3)gene of Guangxi Bama mini-pig and investigate its expresssion in different tissues of Guangxi Bama mini-pig, in order to lay a foundation for revealing PAQR3 fuction and its role in disease models. 【Method】The PAQR3 gene of Guangxi Bama mini-pig was cloned by RT-PCR, the protein structure of porcine PAQR3 was analyzed based on bioinformatics using online prediction software. The PAQR3 gene expression pattern in different tissues and its expression level after being induced by high fat and high sugar were detected by q RT-PCR. 【Result 】The results showed that, the PAQR3 gene of Guangxi Bama mini-pig was 936 bp in length, encoding 312 amino acids. The phylogenetic tree analysis showed that, and the amino acid sequence of porcine PAQR3 protein shared identity of more than 81% with human(XM_006714106.2),macaque(NM_001257693.1), cattle(XM_010806259.1), canis famliliaris(XM_014109889.1), house mouse(XM_006534874.2), sheep(XM_012180242.1) and chicken(XM_001233720.3). It indicated that Guangxi Bama mini-pig had the closest genetic relationship with macaque, but the most distant genetic relationship with cattle. Furthermore, PAQR3 protein contained conserved Hly IⅡ domain and was hydrophilic protein, with 7 transmembrane helices, and the extended strand accounted for 31.83% in the secondary structure. And PAQR3 protein wasn't secretory protein because it had no signal peptide. The q RT-PCR results showed that, PAQR3 gene was expressed in the different tissues of Guangxi Bama mini-pig, especially in lung at the highest level, but in fat at the lowest level. In addition, after being induced by high fat and high sugar, the expression level of PAQR3 gene was reduced significantly in liver tissues of Guangxi Bama mini-pig(P<0.05). 【Conclusion】Th PAQR3 gene can be expressed widely in different tissues of Guangxi Bama mini-pig, and under high content of cholesterol and triglyceride, body can balance cholesterol content by regulating PAQR3 gene expresssion.

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