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Position: Home > Articles > Cloning and Sequences Analysis of Transcription Factor CBF1 and CBF4 Gene in Arabidopsis thaliana Biotechnology Bulletin 2010,2010 (2) 85-89

拟南芥转录因子CBF1和CBF4基因的克隆与序列分析

作  者:
徐春波;王勇;杨志如;赵海霞;李兴酉
单  位:
内蒙古农业大学生态环境学院;中国农业科学院草原研究所;内蒙古鄂尔多斯市水利勘测设计院
关键词:
CBF1;CBF4;拟南芥;PCR;克隆
摘  要:
利用聚合酶链式反应技术(PCR)从拟南芥(Arabidopsis thaliana)植株中扩增出CBF1和CBF4基因,并将其连接到pGEM-Tvector载体上。PCR和酶切鉴定后进行基因测序,结果表明所克隆的CBF1和CBF4基因序列与GenBank中基因序列同源性分别可达99.84%和99.41%,说明这两个片段确为拟南芥CBF1和CBF4基因。
译  名:
Cloning and Sequences Analysis of Transcription Factor CBF1 and CBF4 Gene in Arabidopsis thaliana
作  者:
Xu Chunbo1 Wang Yong2 Yang Zhiru3 Zhao Haixia1 Li Xingyou1(1Grassland Research Institute of Chinese Academy of Agricultural Sciences,Huhhot 010010;2College of Ecology and Environment Inner Mongolia Agricultural University,Huhhot 010018;3 Water Conservancy Survey and Design Institute in Erdos City,Dongsheng 017000)
关键词:
CBF1 CBF4 Arabidopsis thaliana PCR Clone
摘  要:
CBF1 and CBF4 genes were obtained by PCR technique from the plants of Arabidopsis thaliana and then connected those into the pGEM-T vector.PCR,Restriction enzyme digestion and Sequencing results showed that the cloned gene sequences of CBF1 and CBF4 have highly homologous with GenBank,reached 99.84%,99.41% Respectively.Thus,the two fragments were proved as CBF1 and CBF4 gene fragments

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