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Position: Home > Articles > Effect of gossypol on proliferation and apoptosis of primary luteal cells in sow Chinese Journal of Veterinary Science 2009,29 (10) 1303-1306

棉酚对猪原代培养黄体细胞增殖和凋亡的影响

作  者:
龙安梅;邬静;袁莉芸;袁慧
单  位:
湖南农业大学动物医学院
关键词:
醋酸棉酚;猪;原代培养;黄体细胞;细胞增殖;凋亡
摘  要:
采用长大杂母猪原代培养的黄体细胞,通过MTT法、DNA Ladder和TUNEL检测法,探讨了0、0.4、2、10、50 mg/L棉酚对猪原代培养黄体细胞增殖和凋亡的影响。结果表明,棉酚分别染毒24、48 h后,与对照组比较,各浓度组对黄体细胞增殖具有明显的抑制作用(P<0.05,P<0.01),黄体细胞的凋亡率达5%~23%;这种细胞毒性作用呈剂量相关性,在一定剂量下,具有时间依赖关系。
译  名:
Effect of gossypol on proliferation and apoptosis of primary luteal cells in sow
作  者:
LONG An-mei,WU Jing,YUAN Li-yun,YUAN Hui*(College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China)
关键词:
gossypol;sow;primary culture;luteal cells;cell proliferation;apoptosis
摘  要:
This study was designed to determine the effect of gossypol at 0.4,2,10,50 mg/L on proliferation and apoptosis in primary luteal cells of Landrace-Yorkshire Hybrid sows by MTT and TUNEL assay.The results showed that 24 h and 48 h after treatment with gossypol,obvious inhibition effect on luteal cell proliferation was observed in each concentration group(P<0.05,P<0.01).The apoptosis percentage of luteal cells induced by gossypol was among 5% to 23% in each contamination group.Gossypol at high and low dose could inhibit the proliferation of luteal cells and induce their apoptosis.This role was dose-related,and in a certain dosage,a time-dependent manner.

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