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当前位置: 首页 > 文章 > 麦芽糖浆糖基化改性玉米醇溶蛋白及在胶囊壳中的应用 食品科学 2018 (14) 29-35
Position: Home > Articles > Wet-Heating for Glycosylation of Zein with Maltose Syrup and Application in Capsule Shells FOOD SCIENCE 2018 (14) 29-35

麦芽糖浆糖基化改性玉米醇溶蛋白及在胶囊壳中的应用

作  者：
张慧君;陈又铭;辛德慧;沙迪昕;王文霞;姜宁宁;郭浩;张慧敏
单  位：
齐齐哈尔大学食品与生物工程学院
关键词：
玉米醇溶蛋白;糖基化;机械性能;胶囊壳
摘  要：
利用氨基酸分析仪和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE)确定提取玉米醇溶蛋白试剂,采用麦芽糖浆通过湿法糖基化方法改性玉米醇溶蛋白制备胶囊壳,对改性产物进行机械性能分析。结果表明:以70%乙醇溶液提取的玉米醇溶蛋白做糖基化反应的氨基供体,当玉米醇溶蛋白-麦芽糖浆质量比为11∶1、pH 9、超声功率400 W、加热时间15 min条件下,接枝度达34.96%,抗拉强度为9.22 MPa,约为改性前(4.2 MPa)的2倍。SDS-PAGE糖蛋白染色胶片证明了糖分子和蛋白质分子进行有效的接合。将玉米醇溶蛋白改性产物制成胶囊壳,按照《中华人民共和国药典》(2015版)检测胶囊壳规格外观形态、颜色、尺寸、松紧度、脆碎度、干燥质量损失、灼烧残渣和透明度等性能指标,全部合格。
译  名：
Wet-Heating for Glycosylation of Zein with Maltose Syrup and Application in Capsule Shells
作  者：
ZHANG Huijun;CHEN Youming;XIN Dehui;SHA Dixin;WANG Wenxia;JIANG Ningning;GUO Hao;ZHANG Huimin;College of Food and Biological Engineering, Qiqihar University;
单  位：
ZHANG Huijun%CHEN Youming%XIN Dehui%SHA Dixin%WANG Wenxia%JIANG Ningning%GUO Hao%ZHANG Huimin%College of Food and Biological Engineering, Qiqihar University
关键词：
zein;;glycosylation;;structural characterization;;capsule shells
摘  要：
The main objective of this study was to improve the mechanical properties of zein films by glycosylation for producing plant-derived capsule shells. The results showed that zein, which was extracted with 70% ethanol, was the amino donor of glycosylation by amino acid analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE). The optimal conditions were determined as follows: ratio between zein and maltose syrup(MS), 11:1; pH, 9; ultrasonic power, 400 W; and time, 15 min. The tensile strength(TS) of the films produced under these conditions was 9.22 MPa, which was increased by about 2-fold compared with that(4.2 MPa) of unmodified zein films, and the degree of glycosylation(DG) was 34.96%. The formation of conjugates was verified by SDS-PAGE with both protein staining and carbohydrate staining. The appearance, shape, color, size, tightness, friability, mass loss during drying and ash content of zein-MS capsule shells met the requirements of the Chinese pharmacopoeia(2015 Edition).

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