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Position: Home > Articles > Analysis of Dominant Microbial Species in Cantonese Sausage by Independent Culture and PCR-DGGE Technology FOOD SCIENCE 2013,34 (4) 157-160

传统分离培养结合PCR-DGGE技术分析广式腊肠中优势菌

作者：

谢科;余晓峰;郑海松;宗凯;连英琪;刘国庆

单位：

安徽出入境检疫检验局;合肥工业大学生物与食品工程学院

关键词：

广式腊肠;聚合酶链式反应-变性梯度凝胶电泳;16SrDNA;传统分离培养;优势菌

摘要：

采用传统培养方法结合聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)指纹技术对广式腊肠中微生物种群结构进行研究,分析广式腊肠发酵过程的优势微生物群落。结果表明:在传统分离培养中共得到葡萄球菌19株,乳酸菌12株,通过生理生化鉴定得到4株葡萄球菌(P1～P4)和2株乳酸菌(L1、L2)。DGGE指纹图谱显示广式腊肠混合菌群中有3条条带无对应的纯菌株而纯菌株中有1株在混合菌群的图谱上没有相应的条带。进一步的序列分析和相似性比较得出,P1、P4为木糖葡萄球菌,P2、P3、L1、L2分别为孔氏葡萄球菌、阿尔莱特葡萄球菌、格氏乳球菌、乳杆菌属。确定广式腊肠细菌菌群方面的主要优势菌为腐生葡萄球菌、乳杆菌属、木糖葡萄球菌。传统分离法与PCR-DGGE技术结合能够更有效、更全面地分析广式腊肠中微生物群落结构及优势菌。

译名：

Analysis of Dominant Microbial Species in Cantonese Sausage by Independent Culture and PCR-DGGE Technology

作者：

XIE Ke1,YU Xiao-feng2,ZHENG Hai-song2,ZONG Kai2,LIAN Ying-qi1,LIU Guo-qing1,(1.School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China; 2.Anhui Entry-Exit Inspection and Quarantine Bureau,Hefei 230022,China)

关键词：

antonese sausage;polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE);16S rDNA;traditionally independent culture;predominant bacterial species

摘要：

In order to analyze bacterial community structure in Cantonese sausages and explore the predominant microbial species,traditional culture combined with PCR-DGGE fingerprint technology was applied to investigate bacterial population structure in Cantonese sausages.The results showed that 19 Staphylococcus strains and 12 lactic acid strains were obtained by traditional culture method.Four Staphylococcus strains(named as P1-P4) and two lactic acid strains(named as L1 and L2) were identified by physiological-biochemical characteristics.Three bands of total bacterial DNA in DGGE profile of Cantonese sausages were not recovered by cultivation.Conversely,one bacterial species did not have the band of total bacterial DNA in DGGE fingerprint.Sequence analysis and similarity comparison showed that P1 and P4 were Staphylococcus xylosus,and P2,P3,L1 and L2 were Staphylococcus cohnii,Staphylococcus epidermidis,Lactococcus garvieae and Lactobacillales bacterium.The major predominant bacteria in Cantonese sausages were Staphylococcus saprophyticus,Lactobacillales bacterium and Staphylococcus xylosus.Independent culture combined with PCR-DGGE can be applied to the analysis of bacterial community structure and predominant bacteria in Cantonese sausages effectively,which will provide a theoretical reference for the development of culture starter.

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传统分离培养结合PCR-DGGE技术分析广式腊肠中优势菌

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