中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 多脂鳞伞与滑子蘑抗氧化活性及对小鼠血清中IL-2的影响 食用菌学报 2014 (4) 71-75
Position: Home > Articles > Antioxidant and Murine Interleukin-2 Promoting Activities of Pholiota adiposa and P.microspora Fruit Body Extracts Acta Edulis Fungi 2014 (4) 71-75

多脂鳞伞与滑子蘑抗氧化活性及对小鼠血清中IL-2的影响

作  者：
王晓岩;图力古尔;包海鹰
单  位：
吉林农业大学食药用菌教育部工程研究中心
关键词：
多脂鳞伞;滑子蘑;抗氧化活性;DPPH;ABTS;IL-2
摘  要：
以人工栽培多脂鳞伞(Pholiota adiposa)及滑子蘑(Pholiota microspora)子实体为材料,采用回流提取法分别得到石油醚、二氯甲烷、乙酸乙酯及甲醇提取物,以DPPH与ABTS自由基清除率为指标评价各提取物抗氧化活性,结果表明:多脂鳞伞与滑子蘑不同溶剂的提取物均有清除DPPH自由基能力并在一定范围内呈现量效关系。其中乙酸乙酯提取物对DPPH自由基清除能力最强,多脂鳞伞和滑子蘑乙酸乙酯提取物清除DPPH自由基的IC50值分别为0.29mg/mL和0.21mg/mL;同样乙酸乙酯提取物对于ABTS自由基清除能力最强,在质量浓度为1.0mg/mL时,多脂鳞伞和滑子蘑乙酸乙酯提取物对ABTS自由基清除率分别为60.25%和65.27%。以不同剂量的多脂鳞伞和滑子蘑水提物灌胃小鼠20d,测定血清中IL-2(interleukin-2)的含量,用以评价其免疫功能,结果表明:多脂鳞伞水提物3个剂量组处理的小鼠血清中IL-2表达水平明显高于滑子蘑组及阳性对照组,滑子蘑组的IL-2表达水平稍低于阳性对照组,但均显著高于阴性对照组。
译  名：
Antioxidant and Murine Interleukin-2 Promoting Activities of Pholiota adiposa and P.microspora Fruit Body Extracts
作  者：
WANG Xiaoyan;BAU Tolgor;BAO Haiying;Jilin Agricultural University Engineering Research Center of Edible and Medicinal Fungi,Chinese Education Ministry;Academy of Traditional Chinese Medicine,Jilin Agricultural University;
关键词：
Pholiota adiposa;;Pholiota microspora;;antioxidant activity;;DPPH;;ABTS;;Interleukin-2
摘  要：
Extracts of powdered Pholiota adiposa and P.microspora fruit bodies were prepared by refluxing for 2hwith four different organic solvents(petroleum ether at 40 ℃,dichloromethane at 50 ℃,ethyl acetate at 55 ℃ and methanol at 60 ℃),and associated antioxidant activities were determined using DPPH and ABTS free radical scavenging assays.All the P.adiposa and P. microspora extracts exhibited concentration-dependent DPPH free radical scavenging activity;highest activities were recorded with ethyl acetate extracts of both fungi with IC50 values of 0.29mg/mL and 0.21mg/mL,respectively.Corresponding IC50 values for ABTS-scavenging activity were 0.64 mg/mL and 0.53 mg/mL,respectively.Serum interleukin-2(IL-2)levels in mice administered intragastrically with low(3.125mg/kg),medium(6.25mg/kg)and high(12.5mg/kg)doses of aqueous extracts(65 ℃for 2h)of P.adiposa and P.microspora fruit bodies for 20 days were determined by ELISA.Serum IL-2levels were significantly higher in all cases compared with negative controls,and P.adiposa extracts were significantly more stimulatory than P.microspora extracts at the same concentration.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46