中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 从虎皮鹦鹉粪便中分离产细菌素菌株及其细菌素特性分析 生物技术通报 2010,0 (9) 185-190
Position: Home > Articles > Characteristics of Bacteriocin and Bacteriocin-producing Bacteria Isolated from Melopsittacus undulates' Excrement Biotechnology Bulletin 2010,0 (9) 185-190

从虎皮鹦鹉粪便中分离产细菌素菌株及其细菌素特性分析

作  者：
陈新练;陈仪本
单  位：
中国科学院南海海洋研究所;广东省微生物研究所广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室
关键词：
细菌素;虎皮鹦鹉;干酪乳杆菌;抑菌试验;抑菌效价
摘  要：
从虎皮鹦鹉(Melopsittacus undulatus)的粪便中分离得到一株产广谱细菌素的菌株,其细菌素产物对革兰氏阳性和阴性细菌均有较强抑制作用。经形态学、生理生化反应和16S rDNA分子方法鉴定,该菌株初步鉴定为干酪乳杆菌。用硫酸铵分级沉淀得到的细菌素粗提物,作温度、pH值、蛋白酶的敏感性测定,结果显示该细菌素经100℃处理10 min抑菌活性不变,当pH4.0时,对大肠杆菌和金黄色葡萄球菌的抑菌率分别达到99.06%和86.14%,并且对多种蛋白酶表现出敏感性。与Nisin标准品的抑菌效价对比,得到粗提物的效价为204.87 IU/mL。
译  名：
Characteristics of Bacteriocin and Bacteriocin-producing Bacteria Isolated from Melopsittacus undulates' Excrement
作  者：
Chen Xinlian Chen Yiben (1 South China Sea Institute of Oceanology,Guangzhou 510301;2 Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Provincial Key Laboratory of Applied Microbiology,Guangdong Institute of Microbiology,Guangzhou 510070;3 Graduate University of Chinese Academy of Sciences,Beijing 100049)
关键词：
Bacteriocin Melopsittacus undulates Lactobacillus casei Antibacterial experiment Inhibitive potency
摘  要：
A strain of bacteria which can produces a broad-spectrum bacteriocin was isolated from the excrement of the Melopsittacus undulates.Its product could inhibit not only gram-positive bacteria but also gram-negative bacteria efficiently.Detecting its appearance,physiological and biochemical characteristics and analyzing 16S rDNA gene sequence homology,the strain of bacteria was identified as Lactobacillus casei.The sensitivity of rough bacteriocin to temperature,pH and protease was studied preliminarily.The results showed that inhibitive rate of the rough bacteriocin to Escherichia coli and Staphylococcus aureus was 99.06% and 86.14%,respectively,when the pH is 4.0.Incubating for 10 min at 100℃,its activity was still not affected.The bacteriocin showed sensitivity to several proteases.Comparing with the Nisin standard product's potency,the potency of the rough bacteriocin was 204.87 IU/mL.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46