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当前位置: 首页 > 文章 > 光照胁迫下广叶绣球菌菌丝基因差异表达的初步分析 中国食用菌 2017,36 (5) 60-63
Position: Home > Articles > Preliminary Study on Differentially Expressed Genes of Sparassis latifolia under Light Inducing Edible Fungi of China 2017,36 (5) 60-63

光照胁迫下广叶绣球菌菌丝基因差异表达的初步分析

作  者：
肖冬来;张迪;马璐;王宏雨;林衍铨
单  位：
福建省农业科学院食用菌研究所
关键词：
广叶绣球菌;转录组;GO功能分析;差异表达基因
摘  要：
利用高通量测序技术对黑暗和光照下广叶绣球菌(Sparassis latifolia)菌丝进行RNA-Seq分析,比较光照胁迫下的差异表达基因,并对差异基因进行GO功能分析和KEGG pathway分析。结果表明,光照胁迫下显著性差异表达的基因328个,其中,上调、下调表达的基因数分别为157个和171个。差异表达基因的GO注释表明,生物学过程主要为细胞过程、代谢过程、单一生物过程,而分子功能主要为催化、结合和转运子活性。KEGG pathway功能分析结果表明,差异基因主要集中在次级代谢产物合成、淀粉和蔗糖代谢和谷胱甘肽代谢通路中。
译  名：
Preliminary Study on Differentially Expressed Genes of Sparassis latifolia under Light Inducing
作  者：
XIAO Dong-lai;ZHANG Di;MA Lu;WANG Hong-yu;LIN Yan-quan;Institute of Edible&Medicinal Fungi,Fujian Academy of Agricultural Sciences;
单  位：
Institute of Edible&Medicinal Fungi,Fujian Academy of Agricultural Sciences
关键词：
Sparassis latifolia;;transcriptome;;GO function analysis;;differentially expressed gene
摘  要：
The transcriptomes of Sparassis latifolia mycelium under light or dark inducing were compared using the whole genome sequence of Sparassis latifolia as a reference.Both light and dark inducing mycelium were subjected to RNA sequencing and exposed to GO function and KEGG pathway analysis.The results showed that a total of 328 genes were identified as believable.Among them,157 genes were up-regulated and 171 genes were down-regulated.The GO classification showed that,the differentially expressed genes were mostly enriched in cellular process,metabolic process,single-organism process biological pathway and catalytic,binding,transporter molecular functions.KEGG pathway analysis showed that the differentially expressed genes were involved in biosynthesis of secondary metabolites,starch and sucrose metabolism,glutathione metabolism.

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