当前位置: 首页 > 文章 > 罗非鱼蛋白酶解液的多肽与钙复合物的制备及其抑菌分析 食品科学 2009,30 (20) 198-202
Position: Home > Articles > Preparation and Antibacterial Activity of Chelate Complex of Ca~(2+) and Polypeptides from Tilapia Proteins FOOD SCIENCE 2009,30 (20) 198-202

罗非鱼蛋白酶解液的多肽与钙复合物的制备及其抑菌分析

作  者:
丁利君;危雪如
单  位:
广东工业大学轻工化工学院
关键词:
罗非鱼;钙离子;螯合物;抑菌
摘  要:
采用木瓜蛋白酶和风味酶的复合酶解罗非鱼蛋白,制取富含多肽的酶解液;并将酶解液中的多肽与Ca2+反应制备多肽-钙螯合物,探讨反应液的pH值、反应温度、反应时间、多肽与CaCl2的质量比对螯合反应的影响,并对产物进行抑菌实验。结果表明:多肽液与Ca2+的最佳螯合工艺为反应pH7.13,反应温度29.5℃,反应时间24.05min,多肽与CaCl2的质量比2.68:1,在此最佳条件下钙的螯合率为87.5%;产物对多种微生物,特别是对细菌的生长有良好的抑制作用。
译  名:
Preparation and Antibacterial Activity of Chelate Complex of Ca~(2+) and Polypeptides from Tilapia Proteins
作  者:
DING Li-jun,WEI Xue-ru(Faculty of Light and Chemical Industry, Guangdong University of Technology, Guangzhou 510006, China)
关键词:
tilapia;Ca2+;chelate;antibacterial activity
摘  要:
Hydrolysates rich in polypeptides was obtained from enzymatic hydrolysis of tilapia proteins using papain and subtilisin.A Ca2+ chelate was prepared using the hydrolysates and calcium.Effects of pH, temperature, time, and ratio of hydrolysates to CaCl2 on chelation reaction were explored.Apart from this, the Ca2+ chelate was evaluated for antibacterial activity.Results indicated that the optimal chelation reaction between hydrolysates and CaCl2 conducted for 24.05 min at 29.5 ℃ and pH 7.13 with a polypeptide/calcium mass ratio of 2.68:1 yielded 87.5% of Ca2 + chelating rate.The chelated products exhibited an effective inhibition against a majority of microorganisms, especially against Saccharomyces cerevisiae, Aspergillus niger and bacteria such as Esoherichia coli, Staphylococcus aureus, Bacillus subtilis, Psendomonas fluorescens, and Candida albicans.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊