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Position: Home > Articles > Determination of Purine Compounds in Beer by Reversed-phase Ion Pair Chromatography FOOD SCIENCE 2006,27 (9) 219-222

反相离子对色谱法测定啤酒中的嘌呤类物质

作  者:
林先军;李永仙;李崎;武千钧;董建军;顾国贤
单  位:
江南大学教育部工业生物技术重点实验室;青岛啤酒股份有限公司科研中心
关键词:
啤酒;反相离子对色谱;嘌呤类物质;水解
摘  要:
利用反相离子对色谱法(RP-IPC)对啤酒中的腺嘌呤(Ade)、鸟嘌呤(Gua)、黄嘌呤(Xan)和次黄嘌呤(Hyp)等4种嘌呤类物质的测定方法进行了研究。结果表明,当检测波长254nm,流动相为水:甲醇:冰乙酸:四丁基氢氧化铵为883.5:100:15:1.5(V/V/V/V),流速为1ml/min时,方法精密度为1.41%~2.42%,测得四种嘌呤物质的回收率在91.2%~100.3%之间。啤酒中的含嘌呤类物质需要经高氯酸水解成嘌呤,经优化的水解条件为啤酒:高氯酸为1:2、100℃水解30min。
译  名:
Determination of Purine Compounds in Beer by Reversed-phase Ion Pair Chromatography
作  者:
LIN Xian-jun1,LI Yong-xian1,LI Qi1,*,WU Qian-jun2,DONG Jian-jun2,GU Guo-xian1 (1.Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036, China;2.Center of Research and Development, Tsingtao Brewery Co. Ltd., Qingdao 266061, China)
关键词:
beer;reversed-phase ion pair chromatography;purine;hydrolysis
摘  要:
Four purine bases were separated by reversed-phase ion pair chromatography. The optimized conditions were: 254 nm by UV detector with water: methyl alcohol :acetic actd: tetrabutylammonium hydroxide (883.5:100:15:1.5 respectively) as mobile phase and flow rate at 1ml/min. The average recoveries of four purine bases was 91.2%~100.3%, with the accuracy of the method as RSD of all four purine bases around 1.41%~2.42%. Purine compounds in beer were pre-hydrolyzed to purines by perchlorate acid, with the optimal conditions as beer: perchlorate acid (1:2) for 30min at 100℃.

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