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当前位置: 首页 > 文章 > 牛α-乳清蛋白基因5′调控区多态性研究 中国畜牧兽医 2013,40 (1) 136-140
Position: Home > Articles > Study on the Polymorphisms of LALBA Gene 5′ Regulation Region in Cattle China Animal Husbandry & Veterinary Medicine 2013,40 (1) 136-140

牛α-乳清蛋白基因5′调控区多态性研究

作  者：
杨永强;龚俞;焦仁刚;惠嫣婷;刘若余
单  位：
贵州省畜牧技术推广站;贵州大学动物科学学院
关键词：
贵州荷斯坦奶牛;务川黑牛;LALBA基因;SNP;启动子
摘  要：
试验选择品种差异较大的贵州荷斯坦奶牛和务川黑牛构建不同DNA池,设计1对引物分别扩增2个牛种α-乳清蛋白(alpha-lactalbumin,LALBA)基因5′调控区及第1外显子部分序列总长1126bp。结果表明,LALBA基因5′调控区存在5个SNPs位点:T-114C、C-166T、A-225C、C-344T、T-778C,T-114C仅在务川黑牛表现多态性。生物信息学软件预测LALBA基因核心启动子区及转录因子结合位点,SNP位点导致11个转录因子结合位点消失,其中1个位于核心启动子区,产生5个新的转录因子结合位点。突变前后RNA二级结构发生明显改变,目标序列未发现CpG岛。
译  名：
Study on the Polymorphisms of LALBA Gene 5′ Regulation Region in Cattle
作  者：
YANG Yong-qiang1,GONG Yu2,JIAO Ren-gang2,HUI Yan-ting1,LIU Ruo-yu1(1.Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education,Guizhou Key Laboratory of Animal Genetics,Breeding and Reproduction,College of Animal Sciences,Guizhou University,Guiyang 550025,China;2.Animal Science and Technology Station of Guizhou,Guiyang 550001,China)
关键词：
Guizhou Holstein cow;Wuchuan Black cattle;LALBAgene;SNP;promoter
摘  要：
In order to analyze the polymorphisms of LALBAgene 5′regulation region in cattle.Two cattle breeds(Wuchuan Black cattle and Guizhou Holstein cow)with significant difference in breeds property were selected to construct DNA pools,a total of 1126bp sequences of LALBA5′regulation region and part of exon 1were obtained by designing 1pair specific primers.The results showed that 5single nucleotide polymorphisms(SNPs)were found in the 5′regulation region which included T-114C,C-166T,A-225C,C-344T,T-778Crespectively and T-114C was specific to Wuchuan Black cattle.Furthermore,bioinformatics tools were used to predict the core region of the promoter and identify various transcription factors binding sites.It demonstrated that 5new transcription factors binding sites emerged while 11previous transcription factors binding sites disappeared which included one located in the core region of promoter results from the SNPs found in this study,the remarkable change of secondary structure of RNA was also detected but the CpG islands was not found by using various softwares.

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