当前位置: 首页 > 文章 > 粗柄羊肚菌分子鉴定及羊肚菌属真菌系统发育分析 江苏农业学报 2010,26 (2) 395-399
Position: Home > Articles > Molecular Identification of Morchella crassipes and Phylogenetic Analysis of Morchella Fungi Jiangsu Journal of Agricultural Sciences 2010,26 (2) 395-399

粗柄羊肚菌分子鉴定及羊肚菌属真菌系统发育分析

作  者:
何培新;刘伟
单  位:
郑州轻工业学院食品与生物工程学院
关键词:
ITS序列;粗柄羊肚菌;羊肚菌属;系统发育
摘  要:
鉴定河南省郑州市郊区发生的羊肚菌标本,并分析羊肚菌属真菌的系统发育关系。采用内转录间隔区(Internal transcribed sequence,ITS)序列分析进行分子鉴定,结果表明,郑州市郊区发生的羊肚菌为粗柄羊肚菌(Morchella crassipes),与形态鉴定结果一致;系统发育分析结果表明,羊肚菌属真菌整体上至少可以分为4个类群:第Ⅰ类群包括高羊肚菌(M.elata)、黑脉羊肚菌(M.angusticeps)、肋脉羊肚菌(M.costata)、尖顶羊肚菌(M.conica)和M.gigas等种类;第Ⅱ类群包括羊肚菌(M.esculenta)、粗柄羊肚菌和小海绵羊肚菌(M.spongiola)等;第Ⅲ类群仅包括红褐羊肚菌(M.rufobrunnea)1个种;半开羊肚菌(M.semilibera)被归为第Ⅳ类群,与其他类群进化距离更远。ITS序列分析可以用于羊肚菌属种类鉴定和系统发育分析。
译  名:
Molecular Identification of Morchella crassipes and Phylogenetic Analysis of Morchella Fungi
作  者:
HE Pei-xin,LIU Wei(School of Food and Biological Engineering,Zhengzhou Institute of Light Industry,Zhengzhou 450002,China)
关键词:
internal transcribed sequence;Morchella;Morchella crassipes;phylogeny
摘  要:
To identify a morel sample collected from the suburbs of Zhengzhou,Henan Province and to study the phylogenetic relationships of fungi of genus Morchella,internal transcribed sequence(ITS) analysis was applied.The morel mushroom identified is Morchella crassipes,which is consistent with morphological identification.Phylogenetic analysis reveals all Morchella strains can be divided into four groups.The first group includes M.elata,M.angusticeps,M.costata,M.conica and M.gigas;the second group includes M.esculenta,M.crassipes and M.spongiola;the only species M.rufobrunnea belongs to the third group,and the fourth group,which also includes only one species of M.semilibera,has bigger distance with other groups.The sequence analysis of ITS can be used in species identification and study of phylogenetic relationships of genus Morchella.

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