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Position: Home > Articles > Study on extraction of antioxidation peptides of duck blood with complex enzyme method Science and Technology of Food Industry 2012 (2) 282-286+290

复合酶法提取鸭血抗氧化肽工艺的研究

作  者:
马利华;秦卫东;陈学红;郑建胜
单  位:
徐州工程学院食品学院
关键词:
鸭血;蛋白肽;抗氧化性;响应面优化
摘  要:
以SN-TCA指数和DPPH自由基清除率为指标,采用复合酶(木瓜蛋白酶:中性蛋白酶为2:1),确定制备鸭血抗氧化肽的最佳复合酶解工艺。并在单因素基础上,采用Design-Expert7.0软件设计回归正交实验,用响应面分析法优化各因素及其交互作用的最佳组合。结果表明:温度为55.21℃,时间为1.70h,底物浓度为10.0%,酶用量为0.45AU/g,在此条件下进行实验得出:SN-TCA指数为49.13%,DPPH.清除率为80.75%。
译  名:
Study on extraction of antioxidation peptides of duck blood with complex enzyme method
作  者:
MA Li-hua,QIN Wei-dong*,CHEN Xue-hong,ZHENG Jian-sheng(Food Engineering College of Xuzhou Institute,Xuzhou 221008,China)
关键词:
duck blood;peptides;antioxidation;RSM
摘  要:
In order to ascertain the optimal process for preparing anti-oxidation peptides from duck blood with complex enzyme method,the synergistic effect of complex(papain:neutral protease=2:1)was studied,taking the SN-TCA and clearance rate of DPPH as index.Based on single factor tests,the optimum hydrolysis conditions of anti-oxidation peptides from duck blood were obtained through Box-Benhnken central combination design and RSM.The result showed that the best technology were temperature 55.21℃,time 1.70h,substrate concentration 10.0%,the amount of enzyme 0.45AU/g,SN-TCA 49.13% and the clearance rate of DPPH· 80.75%.

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