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兜唇石斛的组织培养研究

作  者:
杜刚;来天超;杨海英
单  位:
云南民族大学化学与生物技术学院民族药资源化学国家民委-教育部重点实验室
关键词:
兜唇石斛;组织培养;种苗;培养基
摘  要:
以兜唇石斛的成熟蒴果作为外植体,比较不同的培养基、激素等因素对种子发芽及试管苗生根的影响。结果表明:以成熟蒴果为外植体能够诱导成苗,种子萌芽的最适培养基为:MS+NAA 0.5mg/L+6-BA 2.0mg/L+GA 1.0mg/L+香蕉汁150g/L+蔗糖20g/L+琼脂8g/L+活性炭2g/L,适宜的生根培养基为:1/4MS+NAA 2.0mg/L+6-BA 0.5mg/L+香蕉汁150g/L+蔗糖20g/L+琼脂8g/L+活性炭2g/L。
译  名:
Study on the Tissue Culture of Dendrobium aphyllum (Roxb.) C.E.C.Fisch
作  者:
DU Gang,LAI Tian-chao,YANG Hai-ying(Key Laboratory of Ethnic Medicine Resource Chemistry,State Ethnic Affairs Commission and Ministry of Education,College of Chemistry and Biotechnology,Yunnan University of Nationalities,Kunming,Yunnan 650500)
关键词:
Dendrobium aphyllum(Roxb.) C.E.C.Fisch;tissue culture;seedling;medium
摘  要:
Taking mature capsule of Dendrobium aphyllum(Roxb.) C.E.C.Fisch as explant,the effect of different medium and phytohormones on seeds germination and root induction of seedlings of Dendrobiuma phyllum(Roxb.) C.E.C.Fisch.were studied.The results showed that seedlings could be induced from seed.The most appropriate medium for seedling inducing was MS+NAA 0.5mg/L+6-BA 2.0mg/L+GA 1.0mg/L+banana juice 150g/L+sugar 20g/L+ agar 8g/L+activated carbon 2g/L.The most appropriate rooting medium was 1/4MS+NAA 2.0mg/L+6-BA 0.5 mg/L+banana juice 150g/L+sugar 20g/L+agar 8g/L+activated carbon 2g/L.

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