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当前位置: 首页 > 文章 > 多浪羊mtDNA D-loop区遗传多样性及系统发育分析 生物技术进展 2014,4 (06) 429-434
Position: Home > Articles > Genetic Diversity and Phylogeny Analysis of Duolang Sheep Based on Mitochondrial DNA-loop Variation Current Biotechnology 2014,4 (06) 429-434

多浪羊mtDNA D-loop区遗传多样性及系统发育分析

作  者：
王世锋;李静;王玉涛
单  位：
叶尔羌绿洲生态与生物资源研究重点实验室;喀什师范学院生物与地理科学系
关键词：
多浪羊;mtDNA D-loop区;遗传多样性
摘  要：
以多浪羊为研究对象,分析绵羊线粒体D-loop区的遗传多样性,为研究多浪羊的起源和进化历史奠定基础。结果显示,多浪羊线粒体DNA D-loop序列长度为945~1 039 bp,A、T、G和C含量分别为29.4%、27.7%、17.7%和25.1%,其中A+T为57.1%,G+C为42.8%。研究获得了26种单倍型,56个多态位点,其中单一多态位点42个,简约信息位点14个。平均核苷酸差异数k为5.289,核苷酸多样度Pi为0.02 415,核苷酸多样度较低,说明多浪羊的遗传多样性贫乏,应采取重点措施予以保护。另外研究发现多浪羊经历过群体扩张,其母系起源除A、B和C世系外,可能存在D或E世系。
译  名：
Genetic Diversity and Phylogeny Analysis of Duolang Sheep Based on Mitochondrial DNA-loop Variation
作  者：
WANG Shi-feng;LI Jing;WANG Yu-tao;Key Laboratory of Ecology and Biological Resources in Yarkand Oasis,Department of Biologic and Geographic Sciences,Kashgar Normal College;
关键词：
Duolang sheep;;mtDNA D-loop region;;genetic diversity
摘  要：
We used the Duolang sheep as object in this study,researched the genetic diversity of mitochondrial D-loop region of sheep,layed the foundation for the study of the origin and evolution of Duolang sheep. The results showed that the length of mt DNA D-loop is 945 ~ 1 039 bp. The percentage of A,T,G,C was 29. 4%,27. 7%,17. 7% and 25. 1%,respectively. A +T was 57. 1%,G + C was 42. 8%. The study acquired 26 haplotypes,56 polymorphic sites. Single polymorphism sites and concision information sites were 42 and 14 respectively. The average number of nucleotide differences k is 5. 289,nucleotide diversity P is 0. 024 15,which indicated that genetic diversity of Duolang sheep was low and key protection measures should be taken. Furthermore,it was also found that Duolang sheep experienced population expansion,there might be D or E lineage of the matrilineal origin besides A,B and C lineage.

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