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Position: Home > Articles > Identification of Pathogen Causing Mulberry Fruit Rot Disease Science of Sericulture 2020 (1) 26-30

桑椹果腐病的病原菌鉴定

作  者：
聂蓓蓓;梁嘉俊;包立军;董小龙;郑泽林;高鸿鹏;张敏娟;焦锋;苏超
单  位：
关键词：
桑椹;果腐病;层出镰刀菌;致病性检测
摘  要：
为探究桑椹果腐病的发病原因,采用常规组织分离法,从桑椹果腐病病果中分离得到一株真菌菌株,在PDA培养基上菌落呈圆形,可产生白色气生菌丝和紫色色素。根据显微观察结果,大型分生孢子较少,呈纺锤型或镰刀型,稍弯曲,向两端逐渐变尖,具有2～5个隔膜,大小(154～407)μm×(14～31)μm;小型分生孢子较多,一端尖细、一端钝圆,大小(33～95)μm×(20～38)μm,无隔膜或有1个隔膜。通过菌落形态和分生孢子形态观察,初步认为其为层出镰刀菌(Fusarium proliferatum)。利用真菌EF-1α基因和rDNA ITS通用引物进行PCR扩增,对扩增产物进行测序分析,进一步确认桑椹果腐病的病原菌是层出镰刀菌。根据柯赫氏法则鉴定病原菌,发现层出镰刀菌对桑椹具有较强的感染能力。
译  名：
Identification of Pathogen Causing Mulberry Fruit Rot Disease
作  者：
Nie Beibei;Liang Jiajun;Bao Lijun;Dong Xiaolong;Zheng Zelin;Gao Hongpeng;Zhang Minjuan;Jiao Feng;Su Chao;College of Animal Science and Technology,Northwest A & F University;
关键词：
Mulberry fruit;;Fruit rot disease;;Fusarium proliferatum;;Pathogenicity detection
摘  要：
In order to indentify the causes of mulberry fruit rot disease,fungal strain was isolated from rotted mulberry fruit by conventional tissue separation. The colony was round and produced whitish aerial mycelium with purple pigments on the PDA medium. According to microscopic observation,its macroconidia was less in number with 2 to 5 septa,spindle or sickle in shape,slightly curved with sharp ends,whose size was(15 4-40 7)μm×(1 4-3 1)μm. In contrast,its microconidia was more in number with 0 to 1 septa,one tapered end and the other obtuse end,whose size was(3 3-9 5)μm×(2 0-3 8)μm. Based on observation on morphology of colony and conidium,it was preliminarily identified as Fusarium proliferatum. According to sequencing results after PCR amplification by universal primers of EF-1α and rDNA ITS,the pathogen causing mulberry rot disease was confirmed to be F. proliferatum. The pathogen was found to have strong infectivity to mulberry fruits evaluated by Kochs method.

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