当前位置: 首页 > 文章 > 一种有效的葡萄叶片总RNA提取方法 新疆农业科学 2004,41 (6) 456-457
Position: Home > Articles > An Effective Method of Extraction Total RNA from Grape Leaves Xinjiang Agricultural Sciences 2004,41 (6) 456-457

一种有效的葡萄叶片总RNA提取方法

作  者:
鲁晓燕;赵英;孙文英
单  位:
新疆玛纳斯县人事局;新疆石河子大学园艺系
关键词:
葡萄叶片;RNA;提取方法
摘  要:
RNA的提取技术是现代分子生物技术的基础,植物RNA的提取比较困难。实验以新鲜的葡萄叶片为材料,采用SDS、CTAB相结合的方法,进行酚/氯仿的提取,消除了DNA及植物中较多的多糖、多酚等污染,提取到较纯净的总RNA,此方法适合于葡萄叶片总RNA的提取,试剂简单经济,实验结果稳定,重现性强。
译  名:
An Effective Method of Extraction Total RNA from Grape Leaves
作  者:
LU Xiao-yan~1, ZHAO Ying~2 , SUN Wen-ying~1(1.Horticulture Department, Shihhotze University, Shihhotze 832003, China; 2.Manas Personnel Bureau, Manas Xinjiang 832200, China)
关键词:
grape leaves; extract RNA; extraction method
摘  要:
Extraction of RNA is a base of modern moleculor technology.It is difficult to extract RNA of plant. In this experiment ,fresh grape leaves are chosen as materials, SDS,CTAB and phenol/ccl4 are used to extract total RNA, to eliminate pollution from polysaccharide etc. .The method is suitable for extracting total RNA from grape leaves.It is simple, inexpensive, stable and easily repeated.

相似文章

计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊