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当前位置: 首页 > 文章 > 从辣木籽中制备4-α-L-鼠李糖基-异硫氰酸苄酯的工艺优化研究 云南农业大学学报(自然科学) 2019 (2) 303-308
Position: Home > Articles > Optimization of Preparation Technology of 4-[(α-L-Rhamnosyloxy)Benzyl] Isothiocyanate from Moringa Oleifera Seeds Journal of Yunnan Agricultural University(Natural Science) 2019 (2) 303-308

从辣木籽中制备4-α-L-鼠李糖基-异硫氰酸苄酯的工艺优化研究

作  者：
杨扬;贺水莲;唐明霆;戴吴兰;史崇颖;田洋;盛军
单  位：
云南农业大学理学院;云南农业大学食品科学技术学院;云南农业大学云南省生物大数据重点实验室;云南农业大学园林园艺学院
关键词：
辣木籽;4-α-L-鼠李糖基-异硫氰酸苄酯;高效液相色谱法;提纯工艺优化
摘  要：
【目的】探索从辣木籽中酶解提取4-α-L-鼠李糖基-异硫氰酸苄酯(4-[(α-L-rhamnosyloxy) benzyl] isothiocyanate,GMG-ITC)的工艺条件及纯化方法。【方法】通过单因素和正交试验的方法,优化了对脱油辣木籽中硫代葡萄糖苷进行酶解提取,进而以二氯甲烷为溶剂进行萃取及重结晶可获得高纯度的GMG-ITC。【结果】通过高效液相色谱法测定酶解提取液中GMG-ITC含量,筛选出制备辣木籽GMG-ITC的最佳酶解提取工艺为:时间9 h,料液比1∶60 (g∶mL),pH值为5.0,温度30℃。【结论】建立了简化且高效的辣木籽脱油、酶解提取、萃取、重结晶4步新工艺组合,制备得到产率为4.70%、纯度达98%的GMG-ITC。
译  名：
Optimization of Preparation Technology of 4-[(α-L-Rhamnosyloxy)Benzyl] Isothiocyanate from Moringa Oleifera Seeds
作  者：
YANG Yang;HE Shuilian;TANG Mingting;DAI Wulan;SHI Chongying;TIAN Yang;SHENG Jun;Yunnan Key Laboratory of Biomass Big Data, Yunnan Agricultural University;College of Science, Yunnan Agricultural University;College of Landscape and Horticulture, Yunnan Agricultural University;College of Food Science and Technology, Yunnan Agricultural University;
单  位：
YANG Yang%HE Shuilian%TANG Mingting%DAI Wulan%SHI Chongying%TIAN Yang%SHENG Jun%Yunnan Key Laboratory of Biomass Big Data, Yunnan Agricultural University%College of Science, Yunnan Agricultural University%College of Landscape and Horticulture, Yunnan Agricultural University%College of Food Science and Technology, Yunnan Agricultural University
关键词：
Moringa oleifera seeds;;4-[(α-L-rhamnosyloxy)benzyl] isothiocyanate;;HPLC;;purification process optimization
摘  要：
[Purpose]To optimize of enzymatic hydrolysis and purification conditions of 4-[(α-Lrhamnosylbenzyl)] isothiocyanate(GMG-ITC) from Moringa oleifera seeds.[Methods]Through single factor and orthogonal experiments, we optimized the conditions for enzymatic extraction of glucosinolates from the de-oiled M. oleifera seeds. Then, the high purity GMG-ITC by extraction and recrystallization with methylene chloride were obtained.[ Result] An optimum condition for enzymatic hydrolysis was obtained as following: material to solvent ratio 1∶60(g∶mL), temperature30 ℃, reaction time 9 h, pH 5, by HPLC analyzing.[Conclusion]We described a simple and efficient four-step process including de-oiling, enzymatic hydrolysis, extraction and recrystallization to afford GMG-ITC from M. oleifera seeds, an overall yield of 4.70% was achieved with 98% purity.

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