当前位置: 首页 > 文章 > 八角莲的愈伤组织和组培根及野生根状茎的鬼臼毒素含量比较研究 浙江大学学报(农业与生命科学版) 2006,32 (1) 56-59
Position: Home > Articles > Study on the podophyllotoxin in the Dysosma versipellis(Hance)M.Cheng and its Callus' Materials. Journal of Zhejiang University (Agriculture and Life Sciences) 2006,32 (1) 56-59

八角莲的愈伤组织和组培根及野生根状茎的鬼臼毒素含量比较研究

作  者:
潘琦;陈绍瑗;姜维梅;Gilbert Y S Chan;傅承新
单  位:
香港理工大学应用生物与化学系;浙江大学生命科学学院植物系统进化与生物多样性实验室
关键词:
鬼臼毒素;八角莲;HPLC;组织培养;抗肿瘤作用
摘  要:
比较研究了峨眉山野生八角莲根、组培愈伤组织及其诱导根的鬼臼毒素含量,为八角莲的快速繁殖和利用生物技术生产鬼臼毒素提供科学依据.高效液相色谱技术(HPLC)显示组织培养的八角莲愈伤组织和诱导根中均含有鬼臼毒素,分别为0.117%和0.148%,野生品的根中其含量为0.600%.通过组织培养可在不破坏自然资源的前提下快速生产鬼臼毒素,从而为开发鬼臼毒素类抗癌药物提供大量原料.
译  名:
Study on the podophyllotoxin in the Dysosma versipellis(Hance)M.Cheng and its Callus' Materials.
作  者:
PAN Qi~1,CHEN Shao-yuan~1,JIANG Wei-mei~1,Gilbert Y S Chan~2,FU Cheng-xin~(1)(1.Lab of Plant Systematic Evolution and Biodiversity,College of Life Sciences,Zhejiang University,Hangzhou 310029,China;2.Department of Applied Biology and Chemical Technology,Hongkong Polytechnic University,Hongkong,China)
关键词:
podophyllotoxin;Dysosma versipellis(Hance);HPLC;tissue culture;antitumor
摘  要:
In this study,HPLC was used to detect the podophyllotoxin content in three parts of Dysosma versipellis(Hance).The content was 0.60% in the wild root,while in the calli and the induced root from this calli,they were 0.117% and 0.148%,respectively.The results suggest that it is possible to produce podophyllotoxin quickly by tissue culture,and provide much raw material to antitumor drugs with little damage to the natural resources.

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