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Position: Home > Articles > Isozyme Phenotype of Different Tissue in Mytilus edulis Hebei Fisheries 2006 (3) 18-19+61

紫贻贝SOD和POD同工酶的组织特异性研究

作  者:
刘慧慧;黄云;赵淑江
单  位:
温州医学院生命科学学院;浙江海洋学院海洋科学与技术学院
关键词:
贻贝;同工酶;组织特异性
摘  要:
用聚丙烯酰胺垂直电泳对紫贻贝的鳃、性腺、外套膜、足、闭壳肌等5种组织的超氧化物歧化酶(SOD)和过氧化物酶(POD)进行了检测分析。结果表明,SOD在各组织器官中均表达,共表现为5条酶带,酶带a和b在上述五种组织中均出现,酶带c在除鳃以外的其他4个组织中有表达,酶带d则在除外套膜以外的4个组织中表达,酶带e仅在性腺中存在,所有上述酶带在性腺和鳃中活性相对较强;POD在不同组织中表达各不相同,酶带a仅在外套膜中存在,酶带b存在于除外套膜外的4个组织中,酶带c在性腺、闭壳肌和外套膜中表达。从检测结果来看,SOD和POD在紫贻贝体内的表达有一定的组织特异性,这种特异性不仅与各组织的不同生理分工相关,而且也与机体对自身基因表达进行调控相联系。
译  名:
Isozyme Phenotype of Different Tissue in Mytilus edulis
作  者:
Liu Huihui Huang Yun(Marine Science and Technology College of Zhejiang Ocean University,Zhoushan 316004)Zhao Shujiang(Life Science college,Zhejiang Medical University,Wen zhou 325027)
关键词:
Mytilus edulis Linnaeus isozyme tissue specificity
摘  要:
The study takes M.edulis Linnaeus as the material,using Polyacrylamide Gel Electrophoresis to study SOD and POD isoenzymes of the gill,foot,gonad,adductor muscle and mantellum.The result indicates that there 5 phenotypes of SOD be identified: phenotype a and b appears in the five tissues;phenotype c has been expressed in all the tissues except for the gill,but phenotype d has been expressed in all the tissues gill,foot,gonad and adductor muscle;phenotype e appears only in gonad.All these SOD phenotypes expressed strongly in gonad and gill.The expression of POD is diverse from each other: phenotype a appears only in mantellum,phenotype b exists in all the tissue except mantellum,and phenotype c expresses in gonad,adductor muscle and mantellum.This kind of specificity is not only contact to the different physiological cent work,but also contact to the genetic control of the body itself.

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