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闽产石仙桃无菌播种与组培快繁技术研究初报

作  者:
温秀萍;林青青;杨成梓;于虹敏
单  位:
福建中医药大学药学院
关键词:
石仙桃;种子;无菌播种;快繁技术
摘  要:
为构建石仙桃无菌播种与组培快繁技术,对石仙桃的无菌播种与快繁技术进行了相关研究。结果表明:石仙桃种子最佳消毒溶液为0.1%升汞,处理时间为8min;种子萌发最佳基本培养基为N6培养基,N6+1.0mg·L-1 6-BA+1.0mg·L-1 NAA激素组合的培养基对种子萌发也同样合适;不同植物激素组合对石仙桃幼苗生长影响无显著性差异,N6+1.5mg·L-1 6-BA+0.5mg·L-1 NAA效果较好;在N6+0.5mg·L-1 6-BA+1.5mg·L-1 NAA+75g·L-1香蕉培养基上生根率达85%,通过植物组织培养技术,成功构建了石仙桃种子的非共生萌发和快繁技术体系。
译  名:
Preliminary Report on Aseptic Seeding and Tissue Culture of Pholidota chinensis Lindl.
作  者:
WEN Xiu-ping;LIN Qing-qing;YANG Cheng-zi;YU Hong-min;College of Pharmacy,Fujian University of Traditional Chinese Medicine;
关键词:
Pholidota chinensis;;seed;;aseptic seeding;;rapid propagation
摘  要:
In order to build the seed rapid propagation technology of Pholidota chinensis,the aseptic seeding and tissue culture of Pholidota chinensis were studied.The results showed that the disinfection treatment with 0.1% HgCl2 solution for 8min was most suitable for aseptic seeding.The best medium for seed germination was N6 medium,the optimum medium of N6+6-BA 1.0mg·L-1+NAA 1.0mg·L-1 was also suitable for seed germination;Different combinations of 6-BA and NAA didn't show significant difference on the growth of young seedlings of Pholidota chinensis,and the appropriate medium was N6+6-BA 1.5mg·L-1+NAA 0.5mg·L-1.Seedling's rooting rate was 85% on the medium of N6+6-BA 0.5mg·L-1+NAA 1.5mg·L-1+75g·L-1 banana.The system of asymbiotic seed germination and rapid propagation of Pholidota chinensis was constructed successfully by the technology of plant tissue culture.

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