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Position: Home > Articles > Preparation of the Polyclonal Antibodies against Sulfadiazine Journal of Henan Agricultural Sciences 2009,38 (5) 125-128

磺胺嘧啶多克隆抗体的制备

作  者:
杨继飞;职爱民;刘庆堂;邓瑞广;柴书军;张改平
单  位:
河南省农业科学院河南省动物免疫学重点实验室
关键词:
磺胺嘧啶;多克隆抗体;制备
摘  要:
为制备磺胺嘧啶(sulfadiazine,SD)多克隆抗体(pAb),用重氮化方法将SD偶联于载体蛋白BSA和OVA上,合成免疫抗原BSA-SD和包被抗原OVA-SD。用紫外扫描、SDS-PAGE凝胶电泳试验检测,结果表明偶联成功。用BSA-SD免疫新西兰大白兔,间接ELISA测定多克隆抗体效价。阻断ELISA结果显示:SD单克隆抗体(pAb)的IC50达19.19ng/mL,和其他磺胺类药物交叉反应小于等于2.7%。结果表明,获得了高效价和特异的pAb。
译  名:
Preparation of the Polyclonal Antibodies against Sulfadiazine
作  者:
YANG Ji-fei,ZHI Ai-min,LIU Qing-tang,DENG Rui-guang,CHAI Shu-jun,ZHANG Gai-ping*(Henan Provincial Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)
关键词:
Sulfadiazine;Polyclonal antibody;Preparation
摘  要:
To prepare the polyclonal antibodies aginst sulfadiazine,the artificial immunizing antigen BSA-SD and coating antigen OVA-SD obtained by diazotization,and further identified by ultraviolet(UV) and SDS-PAGE.New Zealand white rabbits were immunized with BSA-SD.The titres of polyclonal antibodies were detected by indirect ELISA and blocking ELISA.The results showed that the IC50 reached to 19.19 ng/mL and the cross reaction with other sulfa drugs was less than or equal to 2.7%.This indicated that the SD polyclonal antibodies of high titer,sensitivity and specificity has been generated and made it possible to establish immunoassay of SD residues.

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