中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > UeFuz7在菰黑粉菌二型态转换中的作用 植物病理学报 2019 (2) 203-211
Position: Home > Articles > The function on dimorphic transition of UeFuz7 in Ustilago esculenta Acta Phytopathologica Sinica 2019 (2) 203-211

UeFuz7在菰黑粉菌二型态转换中的作用

作  者：
刘洪磊;于金梦;曹乾超;陈彬倩;陆朝晶;叶子弘;俞晓平;张雅芬
单  位：
四川省食品药品检验检测院微生物检验中心;中国计量大学生命科学学院浙江省生物计量与检验检疫技术重点实验室
关键词：
菰黑粉菌;UeFuz7;二型态转换;融合
摘  要：
菰黑粉菌与茭白植株互作形成膨大肉质茎——茭白,已成为我国第二大水生蔬菜。作为一种二态性真菌,菰黑粉菌的二型态转换与侵染茭白密切相关,对茭白孕茭至关重要。本研究基于菰黑粉菌全基因组序列,采用t Blastn进行同源基因查找,克隆得到了玉米瘤黑粉菌Fuz7的同源基因UeFuz7。该基因c DNA全长1 308 bp,无内含子,编码435个氨基酸,在黑粉菌中相对保守。酵母双杂交实验发现,UeFuz7可与UeKpp2蛋白互作。同时其表达模式分析发现,在菰黑粉菌的二型态转换发生时UeFuz7的相对表达量最高。进一步通过PEG介导的原生质体转化获得UeFuz7突变体,表型分析发现:在二型态转换过程中,UeFuz7突变菌株的接合管形成和菌丝生长能力明显减弱。以上结果表明UeFuz7作用于菰黑粉菌的二型态转换。
译  名：
The function on dimorphic transition of UeFuz7 in Ustilago esculenta
作  者：
LIU Hong-lei;YU Jin-meng;CAO Qian-chao;CHEN Bin-qian;LU Chao-jing;YE Zi-hong;YU Xiao-ping;ZHANG Ya-fen;Zhejiang Provincial Key Laboratory of Biometrology and Inspection &Quarantine,College of Life Sciences,China Jiliang University;M icrobiological Inspection Center,Sichuan Institute for Food and Drug Control;
关键词：
Ustilago esculenta;;UeFuz7;;dimorphic transition;;mating
摘  要：
Jiaobai,the second largest aquatic vegetable in China,was formed because of the interaction of Ustilago esculenta and its host Zizania latifolia. As a dimorphic fungus,the dimorphic transition of U. esculenta is closely related to its early infection,which is very important to formation of Jiaobai. In this study,the Ustilago maydis Fuz7 homologous gene UeFuz7 in U. esculenta was cloned,through homologous gene search by t Blastn based on the whole genome sequence of U. esculenta. The c DNA of UeFuz7 is 1 308 bp,without introns,encoding 435 amino acids,which is relatively conservative in the smut fungi. It was found that UeFuz7 interacted with UeKpp2 by yeast double hybrid experiment. Besides,its expression pattern analysis showed that the relative expression of UeFuz7 was the highest during the dimorphic transition of U. esculenta. Furthermore,PEG mediated protoplast transformation was carried out to construct UeFuz7 mutant,and phenotypic analysis found that UeFuz7 disrupted mutants significantly reduced the ability of conjugation tube formation and hyphal growth during dimorphic transition. The above results indicate that UeFuz7 functions on the dimorphic transition of U. esculenta.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46