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Position: Home > Articles > Alanine-substituted mutant on Gly³⁷³ and Asn³⁷⁵ of Cry1Ai-h-loop 2 causes reduction in both toxicity and binding against Helicoverpa armigera Journal of Integrative Agriculture 2019,18 (5)

Alanine-substituted mutant on Gly³⁷³ and Asn³⁷⁵ of Cry1Ai-h-loop 2 causes reduction in both toxicity and binding against Helicoverpa armigera

作者：

Liu Yu-xiao;Zhou Zi-shan;Liang Ge-mei;Song Fu-ping;Zhang Jie

单位：

Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

关键词：

Bacillus thuringiensis;Cry1Ai;Domain II-loop2;Helicoverpa armigera;binding affinity

摘要：

Cry1Ai-h-loop 2 is a mutant of Cry1Ai constructed by exchanging loop 2 from Cry1Ah protein and shows insecticidal activity against Helicoverpa armigera. The toxicity of Cry1Ai-h-loop 2, in contrast to the very low toxicity of Cry1Ai, is closely associated with the eleven residues in the loop 2 region. To characterize the key sites of loop 2 in Cry1Ai-h-loop 2, alanine-substituted mutants were generated. The toxicity of these mutants against H. armigera indicated that dual-mutant on Gly(373) and Asn(375) caused a significant decrease in toxic activity. ELISA binding and competition binding assays demonstrated that the reduction of toxicity in the mutant of interest was correlated with decreased binding affinity.

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Alanine-substituted mutant on Gly³⁷³ and Asn³⁷⁵ of Cry1Ai-h-loop 2 causes reduction in both toxicity and binding against Helicoverpa armigera

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农业科学学报 (英文)

Alanine-substituted mutant on Gly373 and Asn375 of Cry1Ai-h-loop 2 causes reduction in both toxicity and binding against Helicoverpa armigera

相似文章

所属期刊

农业科学学报 (英文)

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农业科学学报 (英文)

Alanine-substituted mutant on Gly³⁷³ and Asn³⁷⁵ of Cry1Ai-h-loop 2 causes reduction in both toxicity and binding against Helicoverpa armigera