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Position: Home > Articles > Development of reverse transcriptase loop-mediated isothermal amplification for rapid and visualized detection of classical swine fever virus. Animal Husbandry and Feed Science 2010,2 (3)

Development of reverse transcriptase loop-mediated isothermal amplification for rapid and visualized detection of classical swine fever virus.

作者：

Gui‐Rong Qu;Huiming Yang;Na Tang;Jinliang Wang;Shijun Fu;Xie Jin-wen;Yue FuJie;Zhiqiang She

单位：

~1,YANG Hui~2,TANG Na~1,WANG Jin-liang~1,FU Shi-jun~1,XIE Jin-wen~1,YUE Fu-jie~3,SHEN Zhi-qiang~1,2*

关键词：

rt;lamp;csfv;reliable;reverse transcriptase;lamp metho

摘要：

[Objective] To develop a rapid and visualized detection method of classical swine fever virus(CSFV) using reverse transcriptase loop-mediated isothermal amplification(RT-LAMP).[Method] A total of six special primers were designed based on the conserved sequences of CSFV gene.After optimizing,the reaction of RT-LAMP was carried out at 63 °C for 45 min.The RT-LAMP products were analyzed by agarose gel electrophoresis.The sensitivity,specificity and repeatability were verified,respectively.[Result] The RT-LAMP method could be used for detecting CSFV rather than six generic viruses.The sensitivity of RT-LAMP was 100 times higher than that of RT-PCR.The detection of 27 clinical samples by RT-LAMP and RT-PCR showed that RT-LAMP is more reliable and convenient.[Conclusion] The RT-LAMP method is sensitive and reliable for the detection of CSFV.

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Development of reverse transcriptase loop-mediated isothermal amplification for rapid and visualized detection of classical swine fever virus.

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