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当前位置: 首页 > 文章 > 金顶侧耳种质资源的遗传多样性鉴定及农艺性状评价 北方园艺 2018 (11) 151-157
Position: Home > Articles > Identification of Genetic Diversity and Evaluation of Agromomic Traits for the Germplasm Resources of Pleurotus citrinopileatus Northern Horticulture 2018 (11) 151-157

金顶侧耳种质资源的遗传多样性鉴定及农艺性状评价

作  者：
张妍;邬向丽;黄晨阳;高巍
关键词：
金顶侧耳;ISSR标记;农艺性状
摘  要：
以20个金顶侧耳(Pleurotus cituinopileatus)菌株作为研究材料,其中8株为栽培菌株,12株为野生菌株。利用ISSR分子标记技术分析金顶侧耳菌株种内遗传多样性,并对其菌丝长速、生物学效率、子实体形态等多个农艺性状进行评价。结果表明:20个金顶侧耳菌株具有丰富的遗传多样性,10条ISSR引物共扩增出92条条带,平均多态性条带频率为98.91%;在相似度0.65水平上绝大部分菌株按照中国北方和南方来源地不同聚为两大类群,显示较强的地域性。金顶侧耳平板上的菌丝长速与生物学效率(产量)不存在显著相关性,而生物学效率(产量)与采收时间显著负相关,菌盖直径、菌盖厚度及菌柄直径三者显著正相关,亲缘关系较远的菌株的农艺性状差异较大。根据评价结果,筛选出采收时间短、生物学效率高的菌株Pc6、Pc7及菌盖大、菌肉厚的菌株Pc13等携带优势性状菌株,可作为下一步育种研究的亲本材料。
译  名：
Identification of Genetic Diversity and Evaluation of Agromomic Traits for the Germplasm Resources of Pleurotus citrinopileatus
作  者：
ZHANG Yan;WU Xiangli;HUANG Chenyang;GAO Wei;Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences/Key Laboratory of Microbial Resources Collection and Preservation,Ministry of Agriculture;
单  位：
ZHANG Yan%WU Xiangli%HUANG Chenyang%GAO Wei%Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences/Key Laboratory of Microbial Resources Collection and Preservation,Ministry of Agriculture
关键词：
Pleurotus citrinopileatus;;ISSR marker;;agronomic trait
摘  要：
A total of 20 strains were used to study the genetic diversity and agronomic traits of Pleurotus citrinopileatus,i.e.,8 cultivated strains and 12 wild ones.The genetic diversity of these strains was analyzed with ISSR markers,and agronomic traits in terms of mycelium growth rate,yield,earliness and morphology of the fruiting bodies were evaluated.The results showed that,a total of92 bands were generated from 10 ISSR primers,of which 98.91% were polymorphic indicating that the20 strains were highly genetical diverse;the 20 strains were divided into two groups at the genetic similarity coefficient of 0.65.The mycelium growth rate of the 20 strains on PDA plates was uncorrelated with the yield,whereas the yield was significantly correlated with earliness of the production;three traits of the morphology of the fruiting bodies were significantly correlated,i.e.,cap size,cap thickness and stipe thickness.Genetical distant strains showed more differences in agronomic traits.According to the trait data,Pc6,Pc7,Pc13 were selected as superior strains carrying ideal traits,i.e.,short production period,high yield,large and thick cap.

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