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当前位置: 首页 > 文章 > HPLC测定丛生竹中总黄酮及(异)牡荆苷、(异)荭草苷的含量 西部林业科学 2018,47 (3) 86-90,95
Position: Home > Articles > Determination of Total Flavonoids and Vitexin,Isovitexin,Orientin,Isoorientin in Cluster Bamboo by High-performance Liquid Chromatography Journal of West China Forestry Science 2018,47 (3) 86-90,95

HPLC测定丛生竹中总黄酮及(异)牡荆苷、(异)荭草苷的含量

作  者：
华梅;陈剑;毕玮;孔继君;呼延丽;李云琴;杨宇明;王娟
关键词：
丛生竹;勃氏甜龙竹;HPLC;竹叶黄酮;超声提取
摘  要：
以勃氏甜龙竹竹叶为原料,对其中竹叶黄酮的超声提取工艺进行研究,同时采用高效液相色谱法(HPLC) 对勃氏甜龙竹、箬竹、浦竹仔及椅子竹中总黄酮及牡荆苷、异牡荆苷、荭草苷、异荭草苷的含量进行测定.结果表明,勃氏甜龙竹超声提取最佳的工艺为:甲醇作为提取溶剂、浓度为70%、料液比为1 :10 (w:v)、超声时间60min、温度40 ℃.在最佳提取条件下,勃氏甜龙竹中总黄酮得率能达到4.63%.采用高效液相色谱-外标法测定4种丛生竹中总黄酮,含量分别为0.7952%、1.1669%、0.0926%、0.8972%.勃氏甜龙竹、箬竹和椅子竹中均含有牡荆苷、异牡荆苷、荭草苷及异荭草苷,浦竹仔中仅含有牡荆苷和异牡荆苷.分析方法的建立,为丛生竹化学资源的利用奠定基础.
译  名：
Determination of Total Flavonoids and Vitexin,Isovitexin,Orientin,Isoorientin in Cluster Bamboo by High-performance Liquid Chromatography
关键词：
cluster bamboo%Dendrocalamus brandisii (Munro) Kurz%HPLC%bamboo flavonoids%ultrasonic extraction
摘  要：
The extraction technique of bamboo flavonoids from Dendrocalamus brandisii (Munro) Kurz leaves were studied by ultrasonic extraction technology. At the same time, the contents of total flavonoids and vitexin, isovitexin, orientin, isoorientin from Dendrocalamus brandisii (Munro) Kurz, Indocalamus tessellatus (Munro) Keng f., Indosasa hispida McClure and Dendrocalamus bambusoides Hsueh et D.Z.Li were determined by high per-formance liquid chromatography (HPLC).The results showed that the optimum ultrasonic process was methanol as extraction solvent, with 70% concentration, 1:10 (w:v) material ratio, 60 min ultrasonic time, 40℃tem-perature.Under the optimum conditions, the extraction rate of total flavonoids in Dendrocalamus brandisii (Munro) Kurz was 4.63%.The contents of total flavonoids in the four kinds of cluster bamboo were 0.795 2%, 1.166 9%, 0.092 6% and 0.897 2%, respectively. Dendrocalamus brandisii (Munro) Kurz, Indocalamus tessellatus (Mun-ro) Keng f. and Dendrocalamus bambusoides Hsueh et D.Z.Li contain vitexin, isovitexin, orientin and isoorien-tin. Indosasa hispida McClure contains only vitexin and isovite. The establishment of analytical methods laid the foundation for the utilization of chemical resources of cluster bamboo.

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