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当前位置: 首页 > 文章 > 蛋氨酸铬对鲤糖代谢相关酶活性及IR、GLUT2和SGLT基因表达的影响 大连海洋大学学报 2018,33 (3) 316-322
Position: Home > Articles > Effects of chromium methionine on activities of glyco-metabolism-related enzymes and expression of IR,GLUT2 and SGLT genes in common carp Cyprinus carpio Journal of Dalian Ocean University 2018,33 (3) 316-322

蛋氨酸铬对鲤糖代谢相关酶活性及IR、GLUT2和SGLT基因表达的影响

作  者：
崔培;尹帅;孙金辉;程镇燕;白东清;乔秀亭;张宝龙;翟胜利
关键词：
蛋氨酸铬;鲤;糖代谢相关酶;IR基因;GLUT2基因;SGLT基因
摘  要：
为探究蛋氨酸铬 (CrMet) 对鲤Cyprinus carpio糖代谢相关酶活性及糖代谢相关基因表达的影响,以酪蛋白为蛋白源,豆油为脂肪源,配制7组纯化饲料,其中Cr3+水平分别为0 (对照)、0.1、0.2、0.4、0.8、1.6、3.2 mg/kg.选取初始体质量为 (40.95±4.80) g的鲤,随机分为7组,分别投喂7种饲料,每个组设置3个重复,每个重复放60尾鱼,饲养8周后,检测其肝胰脏糖代谢相关酶活性;禁食48 h后再投喂,再投喂0、3、6、12、24、48 h 时检测 Cr3+水平为0 (对照)、0.8、3.2 mg/kg 时鱼肝胰脏 IR、GLUT2和肠道SGLT基因的表达量.结果表明:添加0.8 mg/kg Cr3+组的组己糖激酶 (HK)、丙酮酸激酶(PK)、磷酸果糖激酶 (PFK) 和琥珀酸脱氢酶 (SDH) 活力均显著高于对照组 (P<0.05),磷酸酵式丙酮酸激酶 (PEPCK) 活力显著低于对照组 (P<0.05);而添加Cr3+并未对葡萄糖-6-磷酸脱氢酶 (G6PDH)活力产生显著影响 (P>0.05);投喂24、48 h 时,0.8 mg/kg Cr3+组 IR mRNA 表达量显著高于0、3.2 mg/kg Cr3+组 (P<0.05);投喂3 h时,0.8、3.2 mg/kg Cr3+组GLUT2 mRNA表达量均显著高于对照组 (P<0.05), 12、24、48 h时,0.8 mg/kg Cr3+组GLUT2 mRNA表达量显著高于0、3.2 mg/kg Cr3+组 (P<0.05);而不同Cr3+添加水平对鲤肠道 SGLT mRNA表达量无显著性影响 (P>0.05).研究表明,在饲料中添加CrMet能够提高鲤对糖的利用能力,建议Cr3+添加水平为0.8 mg/kg.
译  名：
Effects of chromium methionine on activities of glyco-metabolism-related enzymes and expression of IR,GLUT2 and SGLT genes in common carp Cyprinus carpio
关键词：
chromium methionine%Cyprinus carpio%glyco-metabolism-related enzyme%IR gene%GLUT2 gene%SGLT gene
摘  要：
A feeding trial was conducted to evaluate the effects of dietary chromium methionine(CrMet) on glyco-metabolism-related enzymes,and expression of IR,GLUT2 and SGLT genes in common carp Cyprinus carpio. Com-mon carp with body weight of(40.95±4.80) g were reared in a rearing system consisting of 21 plastic tanks and fed purified diets containing Cr3+at a dose of 0(control group),0.1,0.2,0.4,0.8,1.6,and 3.2 mg/kg for 8 weeks when activities of glyco-metabolism-related enzymes were tested. IR,and GLUT2 mRNA expression levels in hepa-topancreas and SGLT mRNA expression levels in intestine were analyzed in 0.8 mg/kg group and 3.2 mg/kg group during the refeeding after 48 h fasting. The results showed that the fish fed diets containing 0.8 mg/kg Cr3+had sig-nificantly higher activities of hexokinase(HK),pyruvate kinase(PK),phosphofructose kinase(PFK),and succi-nate dehydrogenase (SDH) and lower phosphopyruvate kinase(PEPCK) activity than the fish in 0 mg/kg group did(P<0.05), without significant difference in G6PDH activity betwween the control and Cr3+supplementation groups(P>0.05). There was significantly higher IR mRNA expression level in 0.8 mg/kg Cr3+group than that in 0 mg/kg group 24 h and 48 h after refeeding(P<0.05), significantly higher GLUT2 mRNA expression level in 0.8 and 3.2 mg/kg Cr3+groups than that in 0 mg/kg group 3 h after refeeding(P<0.05).12,24 and 48 h after refeed-ing,GLUT2 mRNA expression level was significantly higher in 0.8 mg/kg Cr3+group than in 0 and 3.2 mg/kg groups(P<0.05),without significant effect on SGLT mRNA expression level in intestine of common carp fed diets containing differert Cr3+level. The findings indicate that dietary addition of Cr3+leads to significant impact on the carbohydrate utilization of common carp and 0.8 mg/kg is the optimal level of Cr3+in the common carp diet under this experimental condition.

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