中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > MALDI-TOF质谱技术检测豆类作物病原细菌 植物检疫 2015,29 (4) 31-37
Position: Home > Articles > Preliminary analysis and detection of plant pathogenic bacteria in Leguminosae by MALDI-TOF mass spectrometry Plant Quarantine 2015,29 (4) 31-37

MALDI-TOF质谱技术检测豆类作物病原细菌

作  者：
冯建军;龙海;李志锋;吴绍精;李一农;章桂明
单  位：
广西大学农学院;深圳出入境检验检疫局
关键词：
MALDI-TOF MS;植物病原细菌;豆类;检测
摘  要：
本文通过基于基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)技术分析豆类作物上7种主要病原细菌,建立了MALDI-TOF MS分析方法。结果表明:构建的MALDI-TOF质谱检测数据库可以快速、准确地区分和鉴定该作物上的主要病原菌。该分析方法具有属特异性和致病变种特异性,可用于豆类作物主要病原细菌的检测。
译  名：
Preliminary analysis and detection of plant pathogenic bacteria in Leguminosae by MALDI-TOF mass spectrometry
作  者：
Feng Jianjun;Long Hai;Li Zhifeng;Wu Shaojing;Li Yinong;Zhang Guiming;Shenzhen Entry-Exit Inspection and Quarantine Bureau;Shenzhen Key Laboratory of Inspection Research & Development of Alien Pests, Shenzhen Scientific Academy of Inspection and Quarantine;College of Agriculture, Guangxi University;
关键词：
MALDI-TOF MS;;plant pathogenic bacteria;;Leguminosae;;detection
摘  要：
Based on matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS) method for analysis and detection of plant pathogenic bacteria in Leguminosae was developed. The results obtained from 2 strains of Pseudomonas savastanoi pv. glycinea, 2 strains of P. syringae pv. syringae, 2 strains of P. savas tanoi pv. phaseolicola, 2 strains of P. syringae pv. pisi, one strains of Xanthomonas axonopodis pv. glycines, one strains of X. axonopdis. pv. phaseoli, and 2 strains of Curtobacterium flaccumfaciens pv. flaccumfaciens using MALDI-TOF MS, show the developed mass fingerprints database could quickly distinguish the four pathovars of Pseudomonas from each other and from representatives of closely related Xanthomonas, or Curtobacterium genera. Our study suggests that proteomic analysis using MALDI-TOF MS is powerful diagnostic tools for the accurate identification of plant pathogenic bacteria in Leguminosae.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46