中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 两株防治烟草青枯病的烟草根际拮抗菌 中国烟草学报 2020,26 (1) 91-99
Position: Home > Articles > Evaluation of two antagonistic bacteria from tobacco rhizosphere for controlling tobacco bacterial wilt Acta Tabacaria Sinica 2020,26 (1) 91-99

两株防治烟草青枯病的烟草根际拮抗菌

作  者：
张欣悦;罗翠琴;陈小洁;王其;王璐;丁婷
单  位：
安徽农业大学植物保护学院
关键词：
根际细菌;烟草青枯病菌;脂肽;趋化性
摘  要：
【目的】进一步丰富烟草青枯病的生防资源。【方法】采用抑菌圈法和盆栽试验测定分离自烟草根际的细菌菌株GZYCT-4和GZYCT-9对青枯病菌的拮抗活性;运用PCR技术检测拮抗细菌的生防标记基因,利用趋化性试验研究烟草根系分泌物及其所含的各种有机酸对拮抗菌根部定殖的影响。【结果】(1)GZYCT-4和GZYCT-9对烟草青枯病菌均具有较好的抑菌效果,培养48 h 的抑菌圈直径分别为16.64 mm和18.90 mm,盆栽试验表明灌根接种施用GZYCT-4和GZYCT-9可有效防治烟草青枯病。(2)GZYCT-4和GZYCT-9菌株均检测到8个枯草芽孢杆菌生防标记基因,其脂肽类粗提物对烟草青枯病菌的抑菌圈直径分别达到16.24 mm和20.71 mm。(3)GZYCT-4对红花大金元根系分泌物有较强的趋化反应,且红花大金元根系分泌物含有的草酸对GZYCT-4吸引作用较大,而GZYCT-9则对K326根系分泌物有较好的趋化反应,K326根系分泌物所含的柠檬酸对GZYCT-9有较好的吸引作用。【结论】GZYCT-4和GZYCT-9菌株均可有效地防控烟草青枯病,且对不同品种的烟草根系分泌物及其所含的各种有机酸有趋化差异。
译  名：
Evaluation of two antagonistic bacteria from tobacco rhizosphere for controlling tobacco bacterial wilt
作  者：
ZHANG Xinyue;LUO Cuiqin;CHEN Xiaojie;WANG Qi;WANG Lu;DING Ting;School of Plant Protection, Anhui Agricultural University;
关键词：
rhizobacteria;;Ralstonia solanacearum;;lipopetide;;chemotaxis
摘  要：
To explore biological control resources of tobacco bacteria wilt,the antagonistic activities of bacterial strains GZYCT-4 and GZYCT-9 isolated from tobacco rhizosphere bacteriaagainst Ralstonia solanacearum was determined by bacteriostatic cycle method and pot experiment.The biocontrol function genes of strains GZYCT-4 and GZYCT-9 were analyzed by PCR.The effects of various organic acids in tobacco root exudates on the colonization of antagonistic bacteria in tobacco roots were investigated by chemotaxisassay.Results indicated that:(1) GZYCT-4 and GZYCT-9 strains had significantly antagonistic effects on R.solanacearum.The diamater of bacteriostatic circle was 16.64 mm and 18.90 mm after 48 hours of culture,respectively.Pot experiments results showed that inoculation with GZYCT-4 and GZYCT-9 strains could effectively control tobacco bacterial wilt.(2) Eight biocontrol marker genes of Bacillus subtilis were detected in strains GZYCT-4 and GZYCT-9,which indicated that the two strains could produce lipopetide metabolites.Moreover,the lipopetides compounds produced by GZYCT-4 and GZYCT-9 exhibited inhibitory activity on growth of R.solanacearum,with the inhibitory zone diameter of 16.24mm and 20.71 mm,respectively.(3) The GZYCT-4 exhibited the strongest chemotactic response toward the Honghua dajinyuan root exudates,and the oxalic acid produced by the Honghua dajinyuan root exudates had better attraction to GZYCT-4;however,GZYCT-9 exhibited the strongest chemotactic response toward the K326 root exudates,and the citric acid produced by the K326 root exudates had better attraction to GZYCT-9.The GZYCT-4 and GZYCT-9 could significantly enhance the resistance of different tobacco cultivars to bacterial wilt,and they exhibited different chemotactic responses toward tobacco root exudates and organic acids.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46