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Position: Home > Articles > Optimization of Hairy Root Induction by Agrobacterium rhizogene in Physalis pubescens L. Northern Horticulture 2017 (8) 115-119

发根农杆菌诱导毛酸浆毛状根条件的优化

作  者:
任如意;李思璇;金秋;梁仕茹;王珊珊
单  位:
牡丹江师范学院生命科学与技术学院
关键词:
毛酸浆;发根农杆菌;毛状根;诱导率
摘  要:
以4种发根农杆菌A4、C58C1、R1601、ATCC15834为诱导菌株侵染毛酸浆叶片、茎段,研究了发根农杆菌种类、外植体类型、不同预培养时间、不同侵染时间、不同共培养时间和乙酰丁香酮对毛酸浆毛状根诱导率的影响,并用PCR检测rolB基因。结果表明:4种发根农杆菌均能诱导毛酸浆外植体产生毛状根,ATCC15834的诱导率相对较高,叶片优于茎段;最佳侵染时间为6~8min;预培养和共培养时间均为2~3d时诱导率较高;乙酰丁香酮(AS)浓度为100μmol·L~(-1)有利于促进毛状根的产生,经PCR检测证明,rolB基因已整合到毛酸浆毛状根基因组中。
译  名:
Optimization of Hairy Root Induction by Agrobacterium rhizogene in Physalis pubescens L.
作  者:
REN Ruyi;LI Sixuan;JIN Qiu;LIANG Shiru;WANG Shanshan;College of Life Science and Technology,Mudanjiang Normal University;
关键词:
Physalis pubescens L;;Agrobacterium rhizogenes;;hairy root;;induction rate
摘  要:
Agrobacterium rhizogenes strains of A4,C58C1,R1601,ATCC15834were used to infect the explants of Physalis pubescens L.,then the influences of different Agrobactrium rhizogenes species,explants types,different preculture times,different infection times and co-culture times and acetosyringone on hairy root induction rate were researched.Using PCR technique to identify the induced hairy roots.The results showed that Agrobacterium rhizogenes strains of A4,C58C1,R1601,ATCC15834all could induce hairy roots,but inductivity of ATCC15834was higher.The inductivity of leaf was higher than stem.The best induction conditions of hairy roots were preculturing for 2—3days,infecting for 6-8minutes,co-culturing for 2-3days.Acetosyringone 100μmol·L~(-1)would be necessary to upgrade transformation frequencies.PCR detection results indicated that the rolBgene in Ri plasmid of A.rhizogenes had integrated into the genome of P.pubescens and been expressed.

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