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Position: Home > Articles > Studies on the method of measuring the enzyme activity of feed xylanase Acta Agriculturae Zhejiangensis 2004,24 (2)

饲用木聚糖酶活性测定方法的研究

作  者:
费笛波;冯观泉;袁超
单  位:
浙江省农业科学院植物保护与微生物研究所
关键词:
饲用木聚糖酶;测定方法;DNS法
摘  要:
探讨了木聚糖酶活测定中的几个关键因素对酶活测值的影响,并以此为主要依据,建立了应用DNS法测定饲用木聚糖酶活力的方法:在40℃,pH5.0条件下,底物浓度1%,酶促反应时间10min,DNS显色5min,于波长540~550nm处测定吸光度A。
译  名:
Studies on the method of measuring the enzyme activity of feed xylanase
作  者:
FEI Di-bo, FENG Guan-quan, YUAN Chao (Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences,Hangzhou 310021 ,China)
关键词:
feed xylanase; measuring method; DNS method
摘  要:
This study discussed various factors of affecting xylanase activity and a measuring method of feed xylanase by the DNS. The results showed that the absorbance of the hydrolysis products was at the wave length of 540-550 nm with the optimum conditions of reaction temperature of 40℃,pH of 5.0,concentration of substrate of 1%, reaction time of 10 min,and heating time of 5 min.

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