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当前位置: 首页 > 文章 > HPLC法测定桑叶和沙棘叶中科罗索酸的含量 山西农业科学 2014,42 (11) 1169-1171
Position: Home > Articles > Determination of Corosolic Acid in Mulberry Leaves and Sea Buckthorn Leaves by HPLC Journal of Shanxi Agricultural Sciences 2014,42 (11) 1169-1171

HPLC法测定桑叶和沙棘叶中科罗索酸的含量

作  者：
吴哲;李小玉;田晓东;张璐;张琼姝;王斌;张吉科;林美珍;张小民
单  位：
山西大学生命科学学院
关键词：
科罗索酸;桑叶;沙棘叶;HPLC
摘  要：
采用高效液相色谱法(HPLC)测定桑叶和沙棘叶中科罗索酸含量,为桑叶和沙棘叶进一步研究开发提供科学依据。色谱条件:Epic C18(4.6 mm×405 mm,5μm)色谱柱。在流动相甲醇∶0.2磷酸(V/V)为82∶18;流速为1 mL/min;检测波长为216 nm;柱温为30℃条件下测定桑叶和沙棘叶中科罗索酸含量。结果表明,科罗索酸在0.040 6~0.487 2 mg/mL质量浓度范围内,峰面积与浓度有着良好的线性关系;平均回收率为101.12%,相对标准偏差为1.074%(n=6)。HPLC法用于检测桑叶、沙棘叶中科罗索酸含量,简便易行、结果准确、重复性好。
译  名：
Determination of Corosolic Acid in Mulberry Leaves and Sea Buckthorn Leaves by HPLC
作  者：
WU Zhe;LI Xiao-yu;TIAN Xiao-dong;ZHANG Lu;ZHANG Qiong-shu;WANG Bin;ZHANG Ji-ke;LIN Mei-zhen;ZHANG Xiao-min;College of Life Sciences,Shanxi University;
关键词：
corosolic acid;;mulberry leaves;;sea buckthorn leaves;;HPLC
摘  要：
HPLC was used to determine the corosolic acid of mulberry leaves and sea buckthorn leaves in this experiments,it was provided a scientific basis for the mulberry leaves and sea buckthorn leaves in its further research and development. Chromatographic condition: Epic C18(4.6 mm×405 mm,5 μm)column was adopted. The mobile phase was methanol-0.2 phosphoric acid(82∶18).The detection wavelength was set at 216 nm. Flow rate was 1.0 mL/min. Column temperature was 30 ℃. The results showed that the calibration curve of corosolic acid was linear in the range of 0.040 6-0.487 2 mg/mL. The mean recovery of corosolic acid was 101.12%, RSD was 1.074%(n=6). The method was used to detect the corosolic acid of mulberry leaves and sea buckthorn leaves,simple, accurate and good repeatability.

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