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Position: Home > Articles > STUDY ON PREPARATION OF SOYBEAN OLIGO-PEPTIDE BY ENZYME Soybean Science 2004,23 (1) 26-29

酶法制备大豆寡肽的研究

作  者:
陶红;梁歧
单  位:
解放军军需大学军需工程系
关键词:
大豆寡肽;酶水解;分子量;DH
摘  要:
以Promatex为工具酶对大豆分离蛋白进行水解 ,制备大豆寡肽。分别讨论了热处理温度和时间对DH值的影响 ,确定了热处理条件为 90℃、10min。Promatex的水解条件为 :pH值为7.0 ,酶用量为 0 .5 3ml,酶添加量为E/S :10 .6 % ,温度 5 0℃ ,水解 6hr。制取的大豆肽的DH为 2 8.2 3% ,PCL在 2 - 4之间为寡肽。
译  名:
STUDY ON PREPARATION OF SOYBEAN OLIGO-PEPTIDE BY ENZYME
作  者:
Tao Hong Liang Qi (The Quatermaster University of PLA,Changchun 130062)
关键词:
Soybean oligo-peptide; Enzymatic hydrolysis; Molecular weight; The degree of hydrolysis
摘  要:
In this article the oligo-peptide was made by hydrolysis of soybean protein with promatex protease as tool enzyme. The effect of the tempratures and time of heating treatment on DH of the hydrolysis of soybean protein was studied respectively.The condition for heating treatment was 90℃ for 15minutes. The optimum conditions for hydrolysis of soybean protein with promates is pH7.0,temperature:50℃,substrate concentration:5%,enzyme dosage:0.53ml,reaction time:6hr . DH of soybean peptide prepaired can be up to 28.23% at the optimium conditions , and PCL of it is between 2 and 4 .It is the oligo-peptide .

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