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当前位置: 首页 > 文章 > 产黄樟素内生真菌交链孢霉LJX27的发酵条件优化 食品科学 2014,35 (17) 165-169
Position: Home > Articles > Optimization of Fermentation Conditions of Alternaria LJX27 for Safrole Production FOOD SCIENCE 2014,35 (17) 165-169

产黄樟素内生真菌交链孢霉LJX27的发酵条件优化

作  者：
徐洲;尹礼国;张超;魏琴
单  位：
宜宾学院生命科学与食品工程学院;发酵资源与应用四川省高校重点实验室
关键词：
二次通用旋转组合设计;岩桂;黄樟素;发酵条件
摘  要：
以岩桂内生真菌交链孢霉(Alternaria)LJX27为研究对象,通过单因素试验研究各发酵工艺因素对黄樟素产量的影响,在此基础上通过二次通用旋转组合设计和响应面法优化黄樟素发酵工艺参数。结果表明:交链孢霉LJX27在初始pH 8、发酵温度24℃、转速200 r/min、培养5 d的条件下,黄樟素产量最高,达2.13 g/L,比优化前黄樟素的产量提高了67.72%。各因素对黄樟素产量的影响大小为初始pH值>发酵时间>发酵温度>转速。该回归模型可以用于优化黄樟素的发酵条件。
译  名：
Optimization of Fermentation Conditions of Alternaria LJX27 for Safrole Production
作  者：
XU Zhou;YIN Li-guo;ZHANG Chao;WEI Qin;College of Life Science and Food Engineering, Yi Bin University;Key Laboratory of Fermentation Resource and Application of Institutes of Higher Learning in Sichuan Province;
关键词：
quadratic general rotary composite design;;Cinnamomum pauciflorum;;safrole;;fermentation condition
摘  要：
The objective of this study was to optimize fermentation conditions of Alternaria LJX27(an endophytic fungus from Cinnamomum?pauciflorum) for enhanced production of safrole by response surface methodology with quadratic general rotary composite design. The production of safrole was investigated with respect to fermentation conditions including inoculum amount, pH, fermentation temperature, fermentation time and shaking speed. Fermentation experiments performed under the optimal conditions: medium pH 8; temperature, 24 ℃; shaking speed, 200 r/min; and incubation time, 5 d; resulted in maximum production of 2.13 g/L, which was 67.72% higher than that obtained before optimization. Safrole production was affected in decreasing order by initial medium pH, fermentation time, fermentation temperature and shaking speed. Our investigations indicated that the developed regression model was applicable for optimizing the fermentation conditions for safrole production.

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