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当前位置: 首页 > 文章 > 响应面试验优化超声辅助提取金银花叶黄酮工艺及其抗氧化活性 食品科学 2016,37 (6) 13-19
Position: Home > Articles > Ultrasonic-Assisted Extraction and Antioxidation of Flavonoids from Lonicera japonica Thunb. Leaves: Process Optimization by Response Surface Methodology and Antioxidant Activity Evaluation FOOD SCIENCE 2016,37 (6) 13-19

响应面试验优化超声辅助提取金银花叶黄酮工艺及其抗氧化活性

作  者：
罗磊;张冰洁;朱文学;刘云宏;王雅琪;董金龙
单  位：
河南科技大学食品与生物工程学院河南省食品原料工程技术研究中心
关键词：
金银花叶;黄酮;超声辅助提取;二次通用旋转组合设计;抗氧化活性
摘  要：
为提高金银花叶中黄酮类化合物的提取率,在乙醇体积分数、提取温度、液料比和超声功率4个单因素试验的基础上,通过二次通用旋转组合设计试验优化金银花叶黄酮的超声辅助提取工艺条件,并对其体外抗氧化活性进行研究。结果表明,在乙醇体积分数60%、液料比65∶1(m L/g)、提取温度46℃、超声功率250 W的条件下金银花叶黄酮提取率最高,可达15.67%,与模型预测值相符。抗氧化实验结果表明,金银花叶黄酮具有较强的抗氧化能力,其清除超氧阴离子自由基的能力与作用时间呈反比,与提取液质量浓度呈正比;清除羟自由基的IC50值为0.11 mg/m L,是对照品的34倍。
译  名：
Ultrasonic-Assisted Extraction and Antioxidation of Flavonoids from Lonicera japonica Thunb. Leaves: Process Optimization by Response Surface Methodology and Antioxidant Activity Evaluation
作  者：
LUO Lei;ZHANG Bingjie;ZHU Wenxue;LIU Yunhong;WANG Yaqi;DONG Jinlong;Food Materials Engineering Technology Research Center of Henan Province, College of Food and Bioengineering,Henan University of Science and Technology;
关键词：
Lonicera japonica Thunb.leaves;;flavonoid;;ultrasonic-assisted extraction;;quadratic general rotation combination design;;antioxidant capacity
摘  要：
The extraction of flavonoids from leaves of Lonicera japonica Thunb.(L. japonica) was optimized by the combined use of single factor experiments and response surface methodology. For this purpose, a second-order rotation combination experimental design was conducted using ethanol concentration, extraction temperature, ratio of solvent to materials and ultrasonic power as the independent variables. The in vitro antioxidant activity of the obtained flavonoids was studied. Results indicated that the highest yield of flavonoids of 15.67% was achieved when the extraction was carried out with an ultrasonic power of 250 W using 60% ethanol as the extraction solvent at a solvent/solid ratio of 65:1(m L/g), which was in good agreement with the model predicted value. Antioxidant tests showed that the flavonoids extracted from the leaves of L. japonica possessed strong antioxidant ability, and the superoxide anion free radical scavenging capacity was negatively correlated with reaction time but positively correlated to their concentration. The IC50 for scavenging hydroxyl radical was 0.11 mg/m L, a 34-fold increase over that of control.

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