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当前位置: 首页 > 文章 > 荞麦脱落酸不敏感基因序列分析 浙江农业学报 2017,29 (2) 186-192
Position: Home > Articles > Sequence analysis of ABA-insensitive gene of common buckwheat,Fagopyrum esculentum Acta Agriculturae Zhejiangensis 2017,29 (2) 186-192

荞麦脱落酸不敏感基因序列分析

作  者：
汪燕;刘瑶;梁成刚;陈晴晴;石桃雄;陈其皎;孟子烨;陈庆富
单  位：
贵州师范大学;陕西米仓山国家级自然保护区管理局
关键词：
普通荞麦;脱落酸不敏感基因;序列分析;蛋白结构
摘  要：
脱落酸不敏感基因(ABA-insensitive,ABI)是调控植物逆境胁迫应答、种子脱落和休眠等的一类转录因子。生物信息学分析发现,普通荞麦ABI(CL38501)基因c DNA全长为963 bp,编码320个氨基酸,含有典型的b ZIP结构域和ABI5家族保守区Ⅱ,推测该基因可能编码ABI5家族蛋白。与野生种质相比,花蕾期和开花期栽培品种ABA信号转导途径中绝大多数基因表达量上调,其中,ABI基因表达量分别上调4.42和3.66倍。对9个栽培品种和5份野生种质的ABI基因进行PCR扩增,发现14份材料的405个位点中多态位点为30个,9个栽培品种间仅含1个多态位点,5个野生种质间含有7个多态位点,说明普通荞麦ABI基因序列高度保守。栽培品种与野生种质ABI序列存在11个共同氨基酸差异位点,蛋白构象预测发现第32位点和第60位点氨基酸发生置换导致蛋白构象发生变化,表明其可能与栽培驯化过程中荞麦丧失休眠与落粒特性相关。
译  名：
Sequence analysis of ABA-insensitive gene of common buckwheat,Fagopyrum esculentum
作  者：
WANG Yan;LIU Yao;LIANG Chenggang;CHEN Qingqing;SHI Taoxiong;CHEN Qijiao;MENG Ziye;CHEN Qingfu;Research Center of Buckwheat Industry Technology,Guizhou Normal University;Shaanxi Micangshan National Nature Reserve;
关键词：
common buckwheat;;ABA-insensitive gene;;sequence analysis;;protein structure
摘  要：
ABA-insensitive( ABI) is a kind of transcription factors that regulates plant stress response,seed shattering and dormancy. Bioinformatic analysis indicated that the c DNA sequence of common buckwheat ABI( CL38501)gene contained 963 bp,coded 320 amino acids including the b ZIP domain and the conserved domain Ⅱ of ABI5,suggesting that it might encode the ABI5 family protein. The major genes involve in ABA signal transduction pathway were up-regulated in cultivar including ABI that was up-regulated by 4. 42-fold and 3. 66-fold at bud stage and flowering stage,respectively,as compared with the wild germplasm of common buckwheat. A total of 405 sites in ABI gene including 30 polymorphic sites were identified among 9 cultivars and 5 wild germplasms of common buckwheat. Only1 polymorphic site was identified among 9 cultivars,whereas 7 polymorphic sites were identified in 5 wild germplasms. These results indicated that the ABI sequence of common buckwheat was highly conserved. A total of 11 con-sistent polymorphic amino acid sites were identified among cultivar and wild germplasm. The change of protein conformation was found between cultivar and wild germplasm of common buckwheat induced by the amino acid transformation at the 32 nd and 60 th sites of ABI,implying that it might be associated with the loss of seed shattering and dormancy during the long domestication process.

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