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当前位置: 首页 > 文章 > AB-8大孔树脂分离纯化枸骨叶总黄酮的工艺研究 食品科学 2010,31 (12) 76-79
Position: Home > Articles > Using AB-8 Macroporous Resin for the Separation and Purification of Total Flavonoids from Folium Ilicis Cornutae FOOD SCIENCE 2010,31 (12) 76-79

AB-8大孔树脂分离纯化枸骨叶总黄酮的工艺研究

作  者：
刘志祥;曾超珍;张玥
单  位：
中南林业科技大学生命科学与技术学院
关键词：
枸骨叶;总黄酮;AB-8大孔树脂;分离纯化
摘  要：
以静态吸附与解吸为考察指标,利用五因素四水平的正交试验对AB-8型大孔树脂分离纯化枸骨叶总黄酮的工艺进行研究。结果表明,AB-8型大孔树脂对枸骨叶总黄酮有较好的纯化效果,最佳纯化工艺条件为吸附液pH4.5、吸附时间4h、解吸液体积与树脂质量之比为25:1(mL/g)、解吸液为体积分数90%的乙醇、解吸时间2.25h,在此条件下,总黄酮回收率可达90.8%,纯度为33.4%。
译  名：
Using AB-8 Macroporous Resin for the Separation and Purification of Total Flavonoids from Folium Ilicis Cornutae
作  者：
LIU Zhi-xiang,ZENG Chao-zhen,ZHANG Yue(College of Life Science and Technology,Central South University of Forestry and Technology,Changsha 410004,China)
关键词：
Folium Ilicis Cornutae;total flavonoids;AB-8 macroporous resin;separation and purification
摘  要：
The application of AB-8 macroporous resin for the separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae was evaluated in the present study.The static adsorption-desorption process of Folium Ilicis Cornutae total flavonoids on this resin was optimized using single factor and orthogonal array design methods.The results showed that a good separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae was achieved using AB-8 macroporous resin.The optimum conditions for the separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae were found to be:4 h adsorption at pH 4.5 of the sample solution,followed by 2.25 h desorption using 90% ethanol as desorption solvent added at 25-fold volume of resin weight.Under these optimum conditions,the recovery of Folium Ilicis Cornutae total flavonoids reached up to 90.8% and the purity of the final product obtained was 33.4%.

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